作者：Jinlian Gu,Chang Yan,ShunYin,Hao Wu,Chi Liu
单位：哈尔滨师范大学
期刊：Controlled Release
U-118MG(人脑星形胶质母细胞瘤)
活动价：
¥880.00
市场价：¥1200.00
一键复制产品信息
产品规格：
- 1×10^6Cells/T25 (常温)
- 1×10^6Cells/Vial (冻存)
货号：
CL0412
培养条件：
DMEM+10%FBS+1%P/S
培养基：
- 细胞信息
- 注意事项
- STR鉴定
- 引用文献
细胞信息
产品名称
U-118MG
形态
混合细胞样
冻存条件
90%FBS+10%DMSO
别名
U-118MG，U-118-MG，U118-MG，U118MG，u1118mg，118MG
培养条件
DMEM+10%FBS+1%P/S
生长特性
贴壁
换液频率
每周2~3次
生物安全等级
BSL-1
细胞背景
种属
人源
组织来源
脑
肿瘤类型
星形细胞瘤
供体信息
47岁，男性，白种人
背景描述
J. Ponten及其同事在1966年至1969年间从恶性胶质瘤中提取的许多细胞系之一(参见U-87 MG, U-138 MG和U-373 MG)。
遗传背景
同工酶
Me-2, 1, PGM3, 2, PGM1, 2, ES-D, 1, AK-1, 1-2, GLO-1, 1-2, G6PD, B, Phenotype Frequency Product: 0.0001
致瘤性
裸鼠可成瘤
抗原表达
Blood Type A, Rh+, HLA Aw24, A28, B12, Bw47
核型
The line has a near pentaploid chromosome number and a wide range of chromosome number distribution (40% of the cells had numbers ranging from 110 to 115). The following 14 markers were found in most metaphases: t(1p,2p), t(3p,?), t(4p,11q), t(7p,22q), M6, t(9q,?), i(11q)18q t(10q,?), M14, M15, M16, M17 and t(10q,22q), 6 of these were found in some and 10 were seen in one only. Normal chromosomes 7, 8, 12, 19, 20 and 22 had 5 to 6 copies per cell, the x had two copies and the Y was absent.
注意事项
- 该细胞为贴壁细胞，正常传代培养选用胰酶消化，消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响，消化过程中应该注意培养细胞形态的变化，一旦胞质回缩，连接变松散，或有成片浮起的迹象就要立即终止消化，同时注意传代应在细胞处于对数期、未达到汇合状态时进行。
细胞操作
-
U-118MG(人脑星形胶质母细胞瘤)
复苏或冻存细胞到货处理方式 -
U-118MG(人脑星形胶质母细胞瘤)
培养操作步骤 -
U-118MG(人脑星形胶质母细胞瘤)
售后条例
STR位点信息
| Amelogenin | X,Y |
| CSF1PO | 11,12 |
| D1S1656 | 11 |
| D2S441 | 11,15 |
| D2S1338 | 23,25 |
| D3S1358 | 15 |
| D5S818 | 11 |
| D7S820 | 9 |
| D8S1179 | 14,15 |
| D10S1248 | 13 |
| D12S391 | 19,22 |
| D13S317 | 9 (ATCC=HTB-15; Cosmic-CLP=687588; Technion Genomics Center BCF) 9,11 (CLS=300362; PubMed=22570425; PubMed=27582061) |
| D16S539 | 12,13 |
| D18S51 | 13 |
| D19S433 | 13 |
| D21S11 | 27,32.2 |
| D22S1045 | 17 |
| FGA | 18,23 (PubMed=27582061) 23 (ATCC=HTB-15; CLS=300362; Technion Genomics Center BCF; PubMed=22570425) |
| Penta D | 10,13 (ATCC=HTB-15; Technion Genomics Center BCF; PubMed=22570425; PubMed=27582061) 13 (CLS=300362) |
| Penta E | 7 (ATCC=HTB-15; CLS=300362; Technion Genomics Center BCF; PubMed=22570425) 7,13 (PubMed=27582061) |
| TH01 | 6 |
| TPOX | 8 |
| vWA | 18 |
细胞培养最优解
套餐
组合价
组合价
¥1080
¥1568
