5637(人膀胱癌细胞)
¥880.00
- 细胞信息
- 注意事项
- STR鉴定
- 相关产品
细胞信息
产品名称
UM-UC-3
形态
上皮细胞样
冻存条件
90%FBS+10%DMSO
别名
UMUC-3; UM-UC3; UMUC3; UC-3; University of Michigan-Urothelial Carcinoma-3
培养条件
MEM+10%FBS+1%P/S
生长特性
贴壁
换液频率
每周2~3次
生物安全等级
BSL-1
细胞背景
种属
人源
组织来源
膀胱
肿瘤类型
膀胱癌
供体信息
男性欧洲人
背景描述
UM-UC-3 [UMUC3] 是一种上皮样细胞，是从患者的膀胱男性中分离出来的，可用于癌症研究。
遗传背景
致瘤性
yes，Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
核型
This is a hypertriploid human cell line. The modal chromosome number was 80, occurring in 42% of cells. Cells with 78 chromosomes also occurred at a high frequency. The rate of cells with higher ploidies was 2.5%. There were 30 or more marker chromosomes in each cell. They included der(1)t(1;?) (p32;?), ?t(1p5p), i(3q), t(7q14q), ?t(2p3p) and others. The X and N3 had single copy per cell, and others were generally two to three copies per cell.
注意事项
- 该细胞为贴壁细胞，正常传代培养选用胰酶消化，消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响，消化过程中应该注意培养细胞形态的变化，一旦胞质回缩，连接变松散，或有成片浮起的迹象就要立即终止消化，同时注意传代应在细胞处于对数期、未达到汇合状态时进行。
细胞操作
-
UM-UC-3(人膀胱移行细胞癌)
复苏或冻存细胞到货处理方式 -
UM-UC-3(人膀胱移行细胞癌)
培养操作步骤 -
UM-UC-3(人膀胱移行细胞癌)
售后条例
STR位点信息
| Amelogenin | X |
| CSF1PO | 10 (ECACC=96020936; PubMed=25877200; PubMed=27270441) 10,11 (ATCC=CRL-1749; Cosmic-CLP=724838) |
| D2S1338 | 23 |
| D3S1358 | 17,18 |
| D5S818 | 12 |
| D7S820 | 8,9 (ATCC=CRL-1749; Cosmic-CLP=724838; ECACC=96020936; PubMed=25877200) 9 (PubMed=27270441) |
| D8S1179 | 13 |
| D13S317 | 8 |
| D16S539 | 8,9 |
| D18S51 | 14 |
| D19S433 | 14,15 |
| D21S11 | 31 |
| FGA | 20,21 |
| Penta D | 13 |
| Penta E | 12 |
| TH01 | 6,9 |
| TPOX | 10 |
| vWA | 17 |
