磷酸盐缓冲液（PBS）1X
¥48.00
J82(人膀胱移行细胞癌)
活动价：
¥880.00
市场价：¥1200.00
一键复制产品信息
- 细胞信息
- 注意事项
- STR鉴定
细胞信息
产品名称
J82
形态
上皮细胞样
冻存条件
90%FBS+10%DMSO
别名
J-82; J 82; J82COT; J82 COT; J82 CO'T; J82/WT
培养条件
MEM+10%FBS+1%P/S
生长特性
贴壁
换液频率
每周2~3次
生物安全等级
BSL-1
细胞背景
种属
人源
组织来源
膀胱
肿瘤类型
膀胱癌
供体信息
58岁男性欧洲人
背景描述
该细胞源自一名58岁患有膀胱移行细胞癌的白人男性。胞内无桥粒，有大量粗面内质网及突出绒毛，表达ras基因。
遗传背景
同工酶
AK-1, 1；ES-D, 1；G6PD, B；GLO-I, 2；Me-2, 1-2；PGM1, 1；PGM3, 2
致癌基因
Contains the ras (H-ras) oncogene
致瘤性
Yes，in nude mice
抗原表达
HLA A2, Aw32, B5, B12, Cw5, Blood Type A
核型
The cell line is aneuploid human male (XY), with most chromosome counts in the triploid range. However, the chromosome count range is quite broad, extending from hyperdiploid to hexaploid. Normal chromosomes N11 and N20 are under-represented with respect to the other normal chromosomes: altered forms of these two chromosomes are prominent as marker chromosomes. Chromosome N13 tends towards over-representation. Five marker chromosomes are noted: 20q+, 11q+, 8p+, del(1)(q31), 5p+(HSR). One of these, 20q, is identical to marker chromosome M1 of C. O'Toole, et al., Br. J. Cancer 38: 64, 1978. The remainder are not as clearly related to the original marker chromosomes noted by those authors.
注意事项
- 1.细胞贴壁较弱，消化时间偏短，注意控制消化时间；
- 2.注意控制细胞密度，过高或者过低都可能影响细胞状态，不要长时间高密度培养；
- 3.细胞生长较快，注意及时换液及传代；
细胞操作
-
J82(人膀胱移行细胞癌)
复苏或冻存细胞到货处理方式 -
J82(人膀胱移行细胞癌)
培养操作步骤 -
J82(人膀胱移行细胞癌)
售后条例
STR位点信息
| Amelogenin | X,Y |
| CSF1PO | 10,11 |
| D2S1338 | 19 |
| D3S1358 | 16,18 |
| D5S818 | 12,13 |
| D7S820 | 9,11 |
| D8S1179 | 8,13 |
| D13S317 | 10,12 (ATCC=HTB-1; CCRID; Cosmic-CLP=753566; KCLB=30001) 12 (PubMed=27270441) |
| D16S539 | 11,12 |
| D18S51 | 10,12 (ATCC=HTB-1) 12 (PubMed=27270441) |
| D19S433 | 12,13 |
| D21S11 | 30 (CCRID) 30,31 (ATCC=HTB-1; PubMed=11416159) |
| FGA | 20,24 |
| Penta D | 9,13 |
| Penta E | 12,15 |
| TH01 | 9.3 |
| TPOX | 11,12 |
| vWA | 17,18 |
STR鉴定图
细胞培养最优解
套餐
组合价
组合价
¥1080
¥1568
