SW620(人结肠癌细胞)

全部细胞

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活动价：

¥880.00

市场价：¥1200.00

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产品规格：

货号：

CL0305

培养条件：

RPMI-1640+10%FBS+1%P/S

培养基：

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种属
人源
组织来源
大肠
肿瘤类型
结直肠腺癌
供体信息
51岁白人男性
背景描述
SW-620细胞系来自一名患有Dukes-C型结直肠癌的51岁男性的大肠，是结直肠癌的关键研究模型，特别是用于癌症生物标志物、化疗和转移性癌细胞的研究。SW-620细胞是研究细胞凋亡和抗失巢凋亡机制的关键，失巢凋亡是一种对防止转移至关重要的程序性细胞死亡形式。利用SW-620结肠癌细胞的研究已经深入到蛋白质组分析中，以了解不同条件下（如缺氧）的蛋白质组变化。低氧SW620细胞表现出特定的蛋白质组适应，这有助于化疗耐药性。

蛋白表达
keratin
同工酶
ES-D, 1；G6PD, B；PEP-D, 1；PGD, A；PGM1, 2；PGM3, 1
基因表达
carcinoembryonic antigen (CEA) 0.15 ng/106 cells/10 days; transforming growth factor alpha; matrilysin; CSAp negative; and colon antigen 3 negative; keratin positive by immunoperoxidase staining; c-myc; K-ras; H-ras; N-ras; Myb; sis; fos
致癌基因
myc+; myb+; ras+; fos+; sis+; p53+; abl-; ros-; src-
致瘤性
Yes, in nude mice。(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
抗原表达
Blood type A; Rh+
突变信息
There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.
核型
modal number = 50; range = 45 to 53. The stemline chromosome number is hyperdiploid with the 2S component occurring at 12% and 11 marker chromosomes were common to S metaphases. One of the 11 markers [7 (7p; 11p)] was disomic., M1 was probably an HSR but the origin of the chromosome could not be identified.

Amelogenin	X
CSF1PO	13,14
D1S1656	13,14
D2S441	10,15
D2S1338	17,24
D3S1358	16
D5S818	13
D6S1043	11,12
D7S820	8,9
D8S1179	13
D10S1248	13
D12S391	17
D13S317	12
D16S539	9,13 (AddexBio=C0009002/68; ATCC=CCL-227; CCRID; CLS=300466; Cosmic-CLP=905962; ECACC=87051203; PubMed=19372543; PubMed=25877200; PubMed=25926053) 13 (Technion Genomics Center BCF)
D18S51	13
D19S433	13
D21S11	30,30.2
D22S1045	16
FGA	24
Penta D	9,15
Penta E	10
TH01	8
TPOX	11
vWA	16