- 细胞信息
- 注意事项
细胞信息
产品名称
DoHH2
形态
淋巴细胞样
冻存条件
95%FBS+5%DMSO
别名
DOHH2;DoHH-2;DOHH-2
培养条件
RPMI-1640+ 10% FBS+ 1% P/S
生长特性
悬浮
换液频率
2-3次/周
生物安全等级
BSL-1
细胞背景
种属
人源
组织来源
胸腔积液
肿瘤类型
淋巴瘤细胞
供体信息
60Y;Male
背景描述
established from the pleural effusion of a 60-year-old man with refractory immunoblastic B cell lymphoma progressed from follicular centroblastic/centrocytic lymphoma in 1990; cells were described in the literature to carry the t(14;18) leading to IGH-BCL2 fusion gene and to express BCL2 mRNA; the original culture was a mixture of EBV - and EBV + cells, of which the EBV - cells were isolated and clonally expanded. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq).
注意事项
- 该细胞为悬浮细胞,在培养的过程中可以通过补加培养基、半量换液或者全换液的方式补充营养物质。在培养过程中若细胞增殖速度较慢或细胞状态不佳,需尽量减少操作频次,保种细胞稳定生长的环境。细胞培养过程中需要较好的控制细胞密度,一般在2×10^5~1×10^6个/ml。
细胞操作
-
DoHH2(人B细胞淋巴瘤细胞)
复苏或冻存细胞到货处理方式 -
DoHH2(人B细胞淋巴瘤细胞)
培养操作步骤 -
DoHH2(人B细胞淋巴瘤细胞)
售后条例









