Caco-2(人结直肠腺癌细胞)
¥1200.00
- 细胞信息
- 注意事项
细胞信息
产品名称
HS-27A
形态
成纤维细胞样
冻存条件
90%FBS+10%DMSO
别名
HS27A;HS27a
培养条件
RPMI-1640+ 10% FBS+ 1% P/S
生长特性
贴壁
换液频率
2-3次/周
生物安全等级
BSL-2
细胞背景
种属
人源
组织来源
骨头；骨髓；基质
供体信息
30Y;Male
背景描述
HS-27A is a fibroblast cell line that was isolated in (year from History) from the stroma of a 30-year-old White male donor. HS-27A is a subclone of HS-27.HS-27A forms large flattened polygonal shaped cells that exemplify "blanket" cells and maintain numerous intercellular contacts with neighboring cells. Unlike HS-5, HS-27A secretes low levels of growth factors and does not support proliferation of isolated progenitor cells in cocultures. HS-27A expresses relatively high levels of vascular cell adhesion molecule 1 (VCAM-1).
遗传背景
抗原表达
Vascular cell adhesion molecule 1 (CD106, VCAM-1)
注意事项
- 该细胞为贴壁细胞，正常传代培养选用胰酶消化，消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响，消化过程中应该注意培养细胞形态的变化，一旦胞质回缩，连接变松散，或有成片浮起的迹象就要立即终止消化，同时注意传代应在细胞处于对数期、未达到汇合状态时进行。
细胞操作
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HS-27A(人骨髓细胞)
复苏或冻存细胞到货处理方式 -
HS-27A(人骨髓细胞)
培养操作步骤 -
HS-27A(人骨髓细胞)
售后条例
