- 细胞信息
- 注意事项
细胞信息
产品名称
SK-N-DZ
形态
成神经细胞样
冻存条件
90%FBS+10%DMSO
别名
SKNDZ
培养条件
DMEM + 10% FBS+ 1% P/S
生长特性
贴壁
换液频率
2-3次/周
生物安全等级
BSL-1
细胞背景
种属
人源
组织来源
大脑
肿瘤类型
神经母细胞瘤细胞
供体信息
2Y;Female
背景描述
SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma.SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma. Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells. Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins. The cells exhibit moderate MDR1 expression. Retinoic acid induces differentiation in this line.
遗传背景
致瘤性
Yes; Yes, forms tumors in nude mice.
注意事项
- 该细胞为贴壁细胞,正常传代培养选用胰酶消化,消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响,消化过程中应该注意培养细胞形态的变化,一旦胞质回缩,连接变松散,或有成片浮起的迹象就要立即终止消化,同时注意传代应在细胞处于对数期、未达到汇合状态时进行。
细胞操作
-
SK-N-DZ(人神经母细胞瘤细胞)
复苏或冻存细胞到货处理方式 -
SK-N-DZ(人神经母细胞瘤细胞)
培养操作步骤 -
SK-N-DZ(人神经母细胞瘤细胞)
售后条例








