U-2932(人弥漫大B淋巴瘤细胞)

活动价:

¥1600.00

市场价:¥1800.00

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产品规格:
  • 1×10^6Cells/T25 (常温)
  • 1×10^6Cells/Vial (冻存)
货号:

FHC2864-Y

培养条件:

RPMI-1640+ 10% FBS+ 1% P/S

  • 细胞信息
  • 注意事项
  • STR鉴定
  • 引用文献
  • 实验验证
  • 相关产品
  • 文件下载

细胞信息

  • 产品名称

    U-2932

  • 形态

    淋巴母细胞样,圆形,单个细胞,少数聚团

  • 冻存条件

    95%FBS+5%DMSO

  • 别名

    U2932

  • 培养条件

    RPMI-1640+ 10% FBS+ 1% P/S

  • 生长特性

    悬浮

  • 换液频率

    2-3次/周

  • 生物安全等级

    BSL-1

细胞背景

  • 种属

    人源

  • 组织来源

    腹水

  • 肿瘤类型

    淋巴瘤细胞

  • 供体信息

    29Y;Female

  • 背景描述

    established in 1996 from the ascites of a 29-year-old woman with diffuse large B cell lymphoma, who 16 years earlier was diagnosed with advanced stage Hodgkin lymphoma, and relapsed several times after multiple chemo- and radiotharapy regimens to complete remissions; cells were described in the literature to overexpress BCL2, BCL6 and p53; assigned to ABC-like lymphoma subtype (activated B-cell). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq). Also, please note: the cell line U-2932 harbours two distinct subpopulations as visible by surface staining of CD19, CD20, and CD38 (see ref 18133); continuous monitoring of subpopulations is advised to prevent accidental loss during passaging.

注意事项

  • 该细胞为悬浮细胞,在培养的过程中可以通过补加培养基、半量换液或者全换液的方式补充营养物质。在培养过程中若细胞增殖速度较慢或细胞状态不佳,需尽量减少操作频次,保种细胞稳定生长的环境。细胞培养过程中需要较好的控制细胞密度,一般在2×10^5~1×10^6个/ml。

细胞操作