- 细胞信息
- 注意事项
细胞信息
产品名称
U-2932
形态
淋巴母细胞样,圆形,单个细胞,少数聚团
冻存条件
95%FBS+5%DMSO
别名
U2932
培养条件
RPMI-1640+ 10% FBS+ 1% P/S
生长特性
悬浮
换液频率
2-3次/周
生物安全等级
BSL-1
细胞背景
种属
人源
组织来源
腹水
肿瘤类型
淋巴瘤细胞
供体信息
29Y;Female
背景描述
established in 1996 from the ascites of a 29-year-old woman with diffuse large B cell lymphoma, who 16 years earlier was diagnosed with advanced stage Hodgkin lymphoma, and relapsed several times after multiple chemo- and radiotharapy regimens to complete remissions; cells were described in the literature to overexpress BCL2, BCL6 and p53; assigned to ABC-like lymphoma subtype (activated B-cell). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq). Also, please note: the cell line U-2932 harbours two distinct subpopulations as visible by surface staining of CD19, CD20, and CD38 (see ref 18133); continuous monitoring of subpopulations is advised to prevent accidental loss during passaging.
注意事项
- 该细胞为悬浮细胞,在培养的过程中可以通过补加培养基、半量换液或者全换液的方式补充营养物质。在培养过程中若细胞增殖速度较慢或细胞状态不佳,需尽量减少操作频次,保种细胞稳定生长的环境。细胞培养过程中需要较好的控制细胞密度,一般在2×10^5~1×10^6个/ml。
细胞操作
-
U-2932(人弥漫大B淋巴瘤细胞)
复苏或冻存细胞到货处理方式 -
U-2932(人弥漫大B淋巴瘤细胞)
培养操作步骤 -
U-2932(人弥漫大B淋巴瘤细胞)
售后条例








