Caco-2(人结直肠腺癌细胞)
¥1200.00
- 细胞信息
- 注意事项
细胞信息
产品名称
HCC70
形态
上皮细胞样
冻存条件
90%FBS+10%DMSO
别名
HCC-70;HCC 70;HCC0070;Hamon Cancer Center 70
培养条件
RPMI-1640+ 10% FBS+ 1% P/S
生长特性
贴壁
换液频率
2-3次/周
生物安全等级
BSL-1
细胞背景
种属
人源
组织来源
乳腺
肿瘤类型
乳腺导管癌
供体信息
49Y;Female
背景描述
HCC70 is an epithelial cell line isolated from a primary ductal carcinoma from a 49-year-old, Black female in 1992, and the cells took 44 months to establish. HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. However, recent studies performed outside of ATCC have given conflicting results about the status of the estrogen receptor for these cells. The cells are poorly differentiated. The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes. The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors.
遗传背景
基因表达
epithelial glycoprotein 2 (EGP2); cytokeratin 19
注意事项
- 该细胞为贴壁细胞，正常传代培养选用胰酶消化，消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响，消化过程中应该注意培养细胞形态的变化，一旦胞质回缩，连接变松散，或有成片浮起的迹象就要立即终止消化，同时注意传代应在细胞处于对数期、未达到汇合状态时进行。
细胞操作
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HCC70(人乳腺导管癌细胞)
复苏或冻存细胞到货处理方式 -
HCC70(人乳腺导管癌细胞)
培养操作步骤 -
HCC70(人乳腺导管癌细胞)
售后条例
