Caco-2(人结直肠腺癌细胞)
¥1200.00
- 细胞信息
- 注意事项
细胞信息
产品名称
DMS 114
形态
上皮细胞样
冻存条件
90%FBS+10%DMSO
别名
DMS-114;DMS114;Darmouth Medical School 114
培养条件
Waymouth's MB 752/1 [PM151215] + 10% FBS+ 1% P/S
生长特性
贴壁
换液频率
2-3次/周
生物安全等级
BSL-1
细胞背景
种属
人源
组织来源
肺
肿瘤类型
肺癌细胞
供体信息
68Y;Male
背景描述
The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung. The patient had not received prior therapy.The patient had not received prior therapy. The cells express HLA class I and class II antigens. Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin.
遗传背景
基因表达
adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; glucagon; 17 beta estradiol; oxytocin - neurophysin (OT-NP)
致瘤性
Yes; Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
抗原表达
Leu 7 +; My23 +; CD11b +
注意事项
- 该细胞为贴壁细胞，正常传代培养选用胰酶消化，消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响，消化过程中应该注意培养细胞形态的变化，一旦胞质回缩，连接变松散，或有成片浮起的迹象就要立即终止消化，同时注意传代应在细胞处于对数期、未达到汇合状态时进行。
细胞操作
-
DMS 114(人肺癌细胞)
复苏或冻存细胞到货处理方式 -
DMS 114(人肺癌细胞)
培养操作步骤 -
DMS 114(人肺癌细胞)
售后条例
