磷酸盐缓冲液（PBS）1X
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HBL-100(人整合SV40基因的乳腺上皮细胞)
活动价：
¥880.00
市场价：¥1200.00
一键复制产品信息
产品规格：
- 1×10^6Cells/T25 (常温)
- 1×10^6Cells/Vial (冻存)
货号：
CL0866
培养条件：
DMEM+10%FBS+1%P/S
培养基：
- 细胞信息
- 注意事项
- STR鉴定
细胞信息
产品名称
HBL-100
形态
上皮细胞样
冻存条件
90%FBS+10%DMSO
别名
HBL 100; HBL100
培养条件
DMEM+10%FBS+1%P/S
生长特性
贴壁
换液频率
每周2~3次
生物安全等级
BSL-2
细胞背景
种属
人源
组织来源
乳腺
供体信息
27岁，女性，白种人
背景描述
该细胞由E.V.Gaffney及其同事从一位没有乳癌家族史的供者乳汁中建立，培养出来的细胞染色体组型在第7代时就不正常；电镜照片显示有微丝、张力原纤维和桥粒；Southern转移表明有整合型SV40病毒基因，不可以当作正常细胞。
遗传背景
同工酶
G6PD, B, PGM1, 1, PGM3, 2, ES-D, 1, Me-2, 0, GLO-1, 2, AK-1, 1-2, Phenotype Frequency Product: 0.0008
致瘤性
裸鼠中可成瘤
抗原表达
HLA A1, A10, A11, B7, B8
病毒
The cells contain a tamdemly integrated SV40 genome it has been reported that they may contain a type D retrovirus that is similar or identical to Mason-Pfizer monkey virus (MPMV).
逆转录酶
阳性
核型
The stemline chromosome number is near triploid with the modal number of 67 chromosomes, and the 2S component occurring at 0.6%. Most chromosome complements consist of about 39 normal and 28 marker chromosomes. Markers such as 2q, 11q+, 11q, t(2q.12), t(2q.5q?), t(6p?.16), 16pt and many others are common to most metaphases. Normal chromosomes 11, 14, 15 and 16 are absent. 2, 12, 17 and 19 are monosomic, and the x is disomic. DNA profiling for amelogenin, a sex-chromosome-specific PCR assay that can distinguish x chromosome-specific products from Y chromosome-specific products revealed the presence of Y chromosomes in this cell line of putative female origin. Confirmation of the general findings was accomplished by QM staining, C-banding, and FISH, with a whole chromosome paint probe to the human Y chromosome.
倍体
非整倍体
注意事项
- 该细胞为贴壁细胞，正常传代培养选用胰酶消化，消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响，消化过程中应该注意培养细胞形态的变化，一旦胞质回缩，连接变松散，或有成片浮起的迹象就要立即终止消化，同时注意传代应在细胞处于对数期、未达到汇合状态时进行。
细胞操作
-
HBL-100(人整合SV40基因的乳腺上皮细胞)
复苏或冻存细胞到货处理方式 -
HBL-100(人整合SV40基因的乳腺上皮细胞)
培养操作步骤 -
HBL-100(人整合SV40基因的乳腺上皮细胞)
售后条例
STR位点信息
| Amelogenin | X (CCRID; PubMed=25877200; PubMed=28889351) X，Y (ATCC=HTB-124; CLS=300178; PubMed=11416159) |
| CSF1PO | 10 |
| D2S1338 | 18，24 |
| D3S1358 | 14，16 |
| D5S818 | 11，12 |
| D7S820 | 8，12 |
| D8S1179 | 12，15 |
| D13S317 | 12 |
| D16S539 | 9，12 |
| D18S51 | 16 |
| D19S433 | 15 |
| D21S11 | 28，30 |
| FGA | 25 |
| Penta D | 12 |
| Penta E | 7 |
| TH01 | 6，8 |
| TPOX | 8 |
| vWA | 16 |
STR鉴定图
细胞培养最优解
套餐
组合价
组合价
¥1080
¥1568
