Caco-2(人结直肠腺癌细胞)
¥1200.00
SNU-1(人胃癌细胞)
活动价：
¥1200.00
市场价：¥1440.00
一键复制产品信息
产品规格：
- 1×10^6Cells/T25 (常温)
- 1×10^6Cells/Vial (冻存)
货号：
CL0834
培养条件：
RPMI-1640+10%FBS+1%P/S+1%丙酮酸钠+1%谷氨酰胺
培养基：
- 细胞信息
- 注意事项
- STR鉴定
细胞信息
产品名称
SNU-1
形态
上皮细胞样
冻存条件
90%FBS+10%DMSO
别名
SNU1; NCI-SNU-1
培养条件
RPMI-1640+10%FBS+1%P/S+1%丙酮酸钠+1%谷氨酰胺
生长特性
贴壁
换液频率
每周2~3次
生物安全等级
BSL-1
细胞背景
种属
人源
组织来源
胃
肿瘤类型
胃癌
供体信息
44岁亚洲男性
背景描述
SNU-1 是一种表现出上皮形态的细胞系，是从一名 44 岁的亚洲男性癌症患者的胃中分离出来的。本品在癌症研究中有应用。
遗传背景
蛋白表达
Vasoactive intestinal peptide (VIP), expressed
致癌基因
myc+; erb-B2+
致瘤性
Yes, reported colony forming efficiency of 1.9% in semisolid medium.
抗原表达
Blood Type O; Rh +，The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.
核型
SNU-1 is a near-diploid cell line with a modal chromosome number of 47; double minute chromosomes were found in 28% of the cells. They have a reported colony-forming efficiency of 1.9% in semisolid medium and a population doubling time of 26 hours. The cells grow as floating aggregates of round cell clusters. This is a hyperdiploid human cell line with the modal chromosome number of 47 occurring in 94% of cells. Higher ploidies occurred at 42%, which is very high. The karyotype of 47,XY,t(1;4) (q24;q33), +M1 is very uniform and stable. M1 is an F- sized small metacentric chromosome.
注意事项
- 该细胞为贴壁细胞，正常传代培养选用胰酶消化，消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响，消化过程中应该注意培养细胞形态的变化，一旦胞质回缩，连接变松散，或有成片浮起的迹象就要立即终止消化，同时注意传代应在细胞处于对数期、未达到汇合状态时进行。
细胞操作
-
SNU-1(人胃癌细胞)
复苏或冻存细胞到货处理方式 -
SNU-1(人胃癌细胞)
培养操作步骤 -
SNU-1(人胃癌细胞)
售后条例
STR位点信息
| Amelogenin | X,Y |
| CSF1PO | 12,13 (ATCC=CRL-5971; Cosmic-CLP=908444) 12,14 (KCLB=00001) |
| D2S1338 | 23,24 |
| D3S1358 | 16 (ATCC=CRL-5971) 16,17 (KCLB=00001) |
| D5S818 | 12,13 (ATCC=CRL-5971; Cosmic-CLP=908444) 13 (KCLB=00001) |
| D7S820 | 9,12 |
| D8S1179 | 13,14 |
| D13S317 | 11,14 (ATCC=CRL-5971; Cosmic-CLP=908444) 12,14 (KCLB=00001) |
| D16S539 | 9,12 |
| D18S51 | 14,22 |
| D19S433 | 13,14.2 |
| D21S11 | 29,32.2 |
| FGA | 22 (KCLB=00001) 22,23 (ATCC=CRL-5971) |
| Penta D | 10,11 |
| Penta E | 11,14 |
| TH01 | 8,9.3 |
| TPOX | 8,10 |
| vWA | 14 |
STR鉴定图
