磷酸盐缓冲液（PBS）1X
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NCI-H520(人肺鳞癌细胞)
活动价：
¥880.00
市场价：¥1200.00
一键复制产品信息
产品规格：
- 1×10^6Cells/T25 (常温)
- 1×10^6Cells/Vial (冻存)
货号：
CL0820
培养条件：
RPMI-1640+10%FBS+1%P/S
培养基：
- 细胞信息
- 注意事项
- STR鉴定
细胞信息
产品名称
NCI-H520
形态
上皮细胞样
冻存条件
90%FBS+10%DMSO
别名
H520; H-520; NCI-HUT-520; NCIH520
培养条件
RPMI-1640+10%FBS+1%P/S
生长特性
贴壁
换液频率
每周2~3次
生物安全等级
BSL-1
细胞背景
种属
人源
组织来源
肺
肿瘤类型
鳞状细胞癌
背景描述
该细胞系于 1982 年从 A.F. Gazdar 从肺鳞状细胞癌患者身上采集的肺肿块样本中建立。与正常肺组织相比，该细胞系表达的 p53 mRNA 水平大大降低。这些细胞没有表现出明显的结构DNA异常。细胞的角蛋白和波形蛋白染色呈阳性，但神经丝三联体蛋白呈阴性。细胞可以在有/没有血清的软琼脂中形成集落。
遗传背景
蛋白表达
The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.
同工酶
AK-1, 1；ES-D, 1；G6PD, B；GLO-I, 2；Me-2, 0；PGM1, 1；PGM3, 1
基因表达
The cells express greatly reduced levels of p53 mRNA relative to normal lung tissue, but exhibit no gross structural DNA abnormalities.
致瘤性
Yes, in nude mice inoculated subcutaneously with 1*10^7 cells (Tumors developed within 21 days at 100% frequency (5/5)).
核型
This is a hypotriploid human cell line. The modal chromosome number is 58 occurring at 30%. The frequency of higher ploidies was 3.2%. Over 30 marker chromosomes were common to all cells, and four others were found in some cells. Among the common markers were 1q+, t(1q8q), 2q+, der(16)t(3;16)(q21;q22), der(19)t(13;19)(q21;q13), and der(5)t(5;5)(p15pq13). Generally, there were two copies of der(5) in each cell. Normal Y and D group chromosomes were absent, and the X chromosome was single.
注意事项
- 1、该细胞对血清要求比较高，建议使用来源进口优质血清
- 2、细胞生长偏慢，注意控制细胞密度不要过低
细胞操作
-
NCI-H520(人肺鳞癌细胞)
复苏或冻存细胞到货处理方式 -
NCI-H520(人肺鳞癌细胞)
培养操作步骤 -
NCI-H520(人肺鳞癌细胞)
售后条例
STR位点信息
| Amelogenin | X |
| CSF1PO | 10 |
| D1S1656 | 14,16.3 |
| D2S441 | 10,11 |
| D2S1338 | 18,23 |
| D3S1358 | 16 |
| D5S818 | 12 (PubMed=25877200) 12,13 (AddexBio=C0016010/4915; ATCC=HTB-182; Cosmic-CLP=908443; Technion Genomics Center BCF) |
| D7S820 | 8,12 |
| D8S1179 | 14,16,17 |
| D10S1248 | 13 |
| D12S391 | 21 |
| D13S317 | 10,11 |
| D16S539 | 8,13 |
| D18S51 | 17 |
| D19S433 | 13,14 |
| D21S11 | 30 |
| D22S1045 | 16 |
| FGA | 22 |
| Penta D | 13 |
| Penta E | 5,14 |
| TH01 | 10 |
| TPOX | 8 |
| vWA | 18,19 |
STR鉴定图
细胞培养最优解
套餐
组合价
组合价
¥1080
¥1568
