769-P(人肾细胞腺癌细胞)
¥1200.00
- 细胞信息
- 注意事项
- 相关产品
细胞信息
产品名称
CV-1
形态
成纤维细胞样
冻存条件
90%FBS+10%DMSO
别名
Cv-1; CV 1; CV-1.K; CV1
培养条件
DMEM+10%FBS+1%P/S
生长特性
贴壁
换液频率
每周2~3次
生物安全等级
BSL-1
细胞背景
种属
猴源
组织来源
肾
供体信息
141天雄性非洲绿猴
背景描述
CV-1 是 1964 年源自肾脏的非洲绿猴细胞系。这种成纤维细胞样细胞系最初用于专注于致癌性 Rous 肉瘤病毒 （RSV） 转化的研究，广泛用于病毒生产、转染和基因沉默的生物学研究。
遗传背景
病毒易感性
Human poliovirus 1、Herpes simplex virus、Eastern equine encephalitis virus、Western equine encephalitis virus、California encephalitis virus、SV40 virus
病毒耐药
Poliovirus 1, herpes simplex, Eastern equine encephalitis, Western equine encephalitis, California encephalitis, SV40
逆转录酶
Negative
核型
This is a pseudodiploid, male African green monkey cell line. The modal chromosome number was 60, occurring in 48% of cells, and the rate of polyploidy was at 4.4%. Only a few markers were found., Of these M1, a probable deleted N11, was found in all cells examined; M3 of unknown origin was in some cells; and the remaining 2 to 3 others of unknown origins were found only once., N11 was uniformly single copied, and N16 was also single copied in most cells. Both X and Y chromosomes were also detected in every cell.
注意事项
- 该细胞为贴壁细胞，正常传代培养选用胰酶消化，消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响，消化过程中应该注意培养细胞形态的变化，一旦胞质回缩，连接变松散，或有成片浮起的迹象就要立即终止消化，同时注意传代应在细胞处于对数期、未达到汇合状态时进行。
细胞操作
-
CV-1(非洲绿猴肾细胞)
复苏或冻存细胞到货处理方式 -
CV-1(非洲绿猴肾细胞)
培养操作步骤 -
CV-1(非洲绿猴肾细胞)
售后条例
