MDA-MB-453(人乳腺癌细胞)
活动价:
市场价:¥1800.00
- 1×10^6Cells/T25 (常温)
- 1×10^6Cells/Vial (冻存)
CL0668
L-15+10%FBS+1%P/S
- 细胞信息
- 注意事项
- STR鉴定
细胞信息
产品名称
MDA-MB-453
形态
上皮细胞样
冻存条件
90%FBS+10%DMSO
别名
MDA-MB 453; MDA MB 453; MDA-MB453; MDAMB453; MDA-453; MDA453; MD Anderson-Metastatic Breast-453
培养条件
L-15+10%FBS+1%P/S
生长特性
贴壁
换液频率
每周2~3次
生物安全等级
BSL-1
细胞背景
种属
人源
组织来源
乳腺
肿瘤类型
乳腺癌
供体信息
48岁白人女性
背景描述
MDA-MB-453 由 R. Cailleau 等人于 1976 年从一名 48 岁女性乳腺癌转移性乳腺癌患者的积液中衍生出来,累及淋巴结、脑以及胸膜腔和心包腔。
遗传背景
蛋白表达
Fibroblast growth factor (FGF), expressed
同工酶
AK-1, 1;ES-D, 1-2;G6PD, B;GLO-I, 1;PGM1, 1;PGM3, 1
致瘤性
No, in immunosuppressed mice;Yes, in semisolid medium
核型
modal number = 90; range = 87 to 91. The cell line is aneuploid human female, with chromosome counts in the hypo- to near-tetraploid range. Normal chromosomes N11, N13, and N17 are absent, and chromosomes N2, N6, and N12 are clearly under-represented with respect to the copy number of other normal chromosomes, while chromosome N20 tends towards over-representation. A large number of marker chromosomes are present with consistency, many of them with more than one copy. The near-diploid population of cells reported for this culture has been replaced by a tetraploid population.
注意事项
- 1.该细胞培养条件为:L-15+10%FBS+1%P/S,L15培养基不需要5%二氧化碳,使用37度培养箱100%空气培养即可,如果没有条件准备空气气相100%的培养箱的,可以采用不透气密封盖的T25培养瓶来培养,培养过程中每天将细胞拿出培养箱换1-2次空气;
- 2.细胞生长偏慢,注意控制细胞密度不要过低;
细胞操作
-
MDA-MB-453(人乳腺癌细胞)
复苏或冻存细胞到货处理方式 -
MDA-MB-453(人乳腺癌细胞)
培养操作步骤 -
MDA-MB-453(人乳腺癌细胞)
售后条例
STR位点信息
| Amelogenin | X |
| CSF1PO | 10,12 |
| D2S1338 | 23,24 |
| D3S1358 | 15 |
| D5S818 | 11 |
| D7S820 | 10 |
| D8S1179 | 10,12 |
| D13S317 | 12 |
| D16S539 | 9 |
| D18S51 | 15,20 |
| D19S433 | 13,14 |
| D21S11 | 29,31 |
| FGA | 18,23 |
| Penta D | 9,10 |
| Penta E | 11 |
| TH01 | 6 |
| TPOX | 10 |
| vWA | 17,18 |
STR鉴定图















