磷酸盐缓冲液（PBS）1X
¥48.00
MS751(人子宫颈表皮癌细胞)
活动价：
¥1200.00
市场价：¥1440.00
一键复制产品信息
产品规格：
- 1×10^6Cells/T25 (常温)
- 1×10^6Cells/Vial (冻存)
货号：
CL0410
培养条件：
MEM+10%FBS+1%P/S
培养基：
- 细胞信息
- 注意事项
- STR鉴定
细胞信息
产品名称
MS751
形态
上皮细胞样
冻存条件
90%FBS+10%DMSO
别名
MS-751; MS 751
培养条件
MEM+10%FBS+1%P/S
生长特性
贴壁
换液频率
每周2~3次
生物安全等级
BSL-1
细胞背景
种属
人源
组织来源
子宫颈
肿瘤类型
人瘤病毒相关性宫颈鳞状细胞癌
供体信息
47岁白人女性
背景描述
MS751 是一种具有上皮形态的细胞系，是从一名 47 岁的白人女性表皮样癌患者的子宫中分离出来的。本产品在癌症和传染病研究中有应用。
遗传背景
同工酶
AK-1, 1；ES-D, 1；G6PD, B；GLO-I, 1-2；Me-2, 1；PGM1, 1-2；PGM3, 1-2
基因表达
MS751 cells have been reported to contain human papilloma virus 18 (HPV-18) sequences. More recently, it has been shown that MS751 cells contain a partial HPV-45 genome, and that HPV-45 sequences from the E6/E7 region are expressed as poly(A)+ RNA.
致瘤性
Yes, in nude mice; forms poorly differentiated epidermoid carciNoma (grade III).
抗原表达
Blood Type AB; Rh+
核型
model number = 39; range = 38 to 41. This is a hypodiploid human cell line with the modal chromosome number of 39, occurring in 62% of cells. The rate of cells with higher ploidies was 16%. Sixteen markers were found in single copies in all cells. They include del(2) (q34), del(8) (p21), 14p+, der(X)t(X;14) (q28;q13), t(3q8q) and eleven others. A few different markers, all of which had 11qter--11p13 [der(11)] in common, were found. These markers appeared to be mutually exclusive, each cell having one, and only one, marker at a time. Normal X, N8, N11, N21 and N22 were absent. N1, N10, N15, and N16 were paired, and other normal chromosomes were single.
注意事项
- 该细胞为贴壁细胞，正常传代培养选用胰酶消化，消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响，消化过程中应该注意培养细胞形态的变化，一旦胞质回缩，连接变松散，或有成片浮起的迹象就要立即终止消化，同时注意传代应在细胞处于对数期、未达到汇合状态时进行。
细胞操作
-
MS751(人子宫颈表皮癌细胞)
复苏或冻存细胞到货处理方式 -
MS751(人子宫颈表皮癌细胞)
培养操作步骤 -
MS751(人子宫颈表皮癌细胞)
售后条例
STR位点信息
| Amelogenin | X |
| CSF1PO | 11 (CCRID; PubMed=25877200) 11,12 (ATCC=HTB-34; Cosmic-CLP=1240179; DepMap=ACH-001360; PubMed=29156801) |
| D2S1338 | 22 |
| D3S1358 | 19 |
| D5S818 | 12 |
| D7S820 | 9,11 |
| D8S1179 | 14 |
| D13S317 | 12 |
| D16S539 | 11 |
| D18S51 | 12 |
| D19S433 | 16 |
| D21S11 | 29,30 |
| FGA | 20 |
| Penta D | 9 |
| Penta E | 12 |
| TH01 | 6 |
| TPOX | 8 |
| vWA | 16 |
STR鉴定图
细胞培养最优解
套餐
组合价
组合价
¥1280
¥2040
