磷酸盐缓冲液（PBS）1X
¥48.00
JAR(人胎盘绒膜癌细胞)
活动价：
¥880.00
市场价：¥1200.00
一键复制产品信息
产品规格：
- 1×10^6Cells/T25 (常温)
- 1×10^6Cells/Vial (冻存)
货号：
CL0398
培养条件：
RPMI-1640+10%FBS+1%P/S
培养基：
- 细胞信息
- 注意事项
- STR鉴定
细胞信息
产品名称
JAR
形态
上皮细胞样
冻存条件
90%FBS+10%DMSO
别名
Jar; JAr; JaR
培养条件
RPMI-1640+10%FBS+1%P/S
生长特性
贴壁
换液频率
每周2~3次
生物安全等级
BSL-1
细胞背景
种属
人源
组织来源
胎盘
肿瘤类型
绒毛膜癌
供体信息
24岁白人女性
背景描述
JAR 系由 R.A. Pattillo 及其同事直接从胎盘滋养细胞肿瘤中建立。
遗传背景
蛋白表达
Estrogen, progesterone, hCG, human chorionic somatomammotropin (placental lactogen), hCG production averages 22.5 ng/ml after reculturing
同工酶
G6PD, B, PGM1, 1-2, PGM3, 1-2, ES-D, 2, AK-1, 1, GLO-1, 1, Phenotype Frequency Product: 0.0002
核型
This is probably a pseudotriploid human cell line with the modal chromosome number of 68, occurring in 24% of cells, but cells with both 69 (22%) and 70 (18%) chromosome counts also occurred frequently. Cells with higher ploidies occurred at 7.0%. Karyotypes were extremely complex. Consistently there were 20 to 25 marker chromosomes (>30% of total chromosome content) per cell. Most marker chromosomes had complex structural rearrangements, and the origin of chromosome segments of these markers often defied identification. Among the frequently found markers were 8p+, 11p, a large metacentric [?t(3qter--3q12::?--C--?::3q12--3qter)] der(?13)T(1;?13) (p13;?q14), and many others. There was only one normal X chromosome. Normal N3 and N13 were not found.
注意事项
- 该细胞为贴壁细胞，正常传代培养选用胰酶消化，消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响，消化过程中应该注意培养细胞形态的变化，一旦胞质回缩，连接变松散，或有成片浮起的迹象就要立即终止消化，同时注意传代应在细胞处于对数期、未达到汇合状态时进行。
细胞操作
-
JAR(人胎盘绒膜癌细胞)
复苏或冻存细胞到货处理方式 -
JAR(人胎盘绒膜癌细胞)
培养操作步骤 -
JAR(人胎盘绒膜癌细胞)
售后条例
STR位点信息
| Amelogenin | X,Y |
| CSF1PO | 7,10 |
| D2S1338 | 20,21 |
| D3S1358 | 14 (CLS=300221) 14,16,17 (DSMZ=ACC-462) 16,17 (ATCC=HTB-144; KCLB=30144) |
| D5S818 | 10,11 |
| D7S820 | 10,11 |
| D8S1179 | 14,16 |
| D13S317 | 11 |
| D16S539 | 9,10 |
| D18S51 | 13,17 (CLS=300221) 14,15 (ATCC=HTB-144; DSMZ=ACC-462) |
| D19S433 | 12,13 |
| D21S11 | 28,29 (ATCC=HTB-144; DSMZ=ACC-462) 30 (CLS=300221) |
| FGA | 22 |
| Penta D | 9,11 (CLS=300221) 9,14 (ATCC=HTB-144; DSMZ=ACC-462) |
| Penta E | 10,12 (CLS=300221) 8,12 (ATCC=HTB-144; DSMZ=ACC-462) |
| TH01 | 6,7 |
| TPOX | 8,11 |
| vWA | 16,18 |
STR鉴定图
细胞培养最优解
套餐
组合价
组合价
¥1080
¥1568
