EMT6(小鼠乳腺细胞)
活动价:
市场价:¥1200.00
- 1×10^6Cells/T25 (常温)
- 1×10^6Cells/Vial (冻存)
CL0363
DMEM+10%FBS+1%P/S
- 细胞信息
- 注意事项
- STR鉴定
细胞信息
产品名称
EMT6
形态
上皮细胞样
冻存条件
90%FBS+10%DMSO
别名
EMT-6; Experimental Mammary Tumour-6
培养条件
DMEM+10%FBS+1%P/S
生长特性
贴壁
换液频率
每周2~3次
生物安全等级
BSL-1
细胞背景
种属
小鼠
组织来源
乳腺
肿瘤类型
乳腺恶性肿瘤
供体信息
BALB/cCRGL.雌性小鼠
背景描述
EMT6 细胞系是一种小鼠乳腺癌细胞系,广泛用于癌症研究,尤其是与乳腺癌相关的研究。EMT6 细胞起源于 BALB/c 小鼠的自发性肿瘤,在体外和体内均用于分析肿瘤发生、转移和化疗耐药性。这些细胞的特点是当移植到免疫功能正常的小鼠中时能够迅速形成肿瘤,使其成为研究肿瘤免疫和抗癌疗法疗效的理想模型。
遗传背景
致瘤性
Yes, forms solid tumors in some sublines of BALB/c mice
核型
This is a human cell line with near-diploid chromosome counts. The modal chromosome number was 47, occurring in 36% of cells. The rate of cells with a higher ploidy count was 3.9%. Twelve markers were common to most cells. Among them were del(1) (q32.1), der (5)t(5;13) (p15.33;q11), i(5p), der(1)t(1;?) (p34.3;?) and der (6)t(6;7) (p25.3;q21.2). All markers were present in single copy per cell. Normal N1 and N6 were absent. There were two normal X chromosomes. No other abnormalities were detected.
注意事项
- 该细胞为贴壁细胞,正常传代培养选用胰酶消化,消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响,消化过程中应该注意培养细胞形态的变化,一旦胞质回缩,连接变松散,或有成片浮起的迹象就要立即终止消化,同时注意传代应在细胞处于对数期、未达到汇合状态时进行。
细胞操作
-
EMT6(小鼠乳腺细胞)
复苏或冻存细胞到货处理方式 -
EMT6(小鼠乳腺细胞)
培养操作步骤 -
EMT6(小鼠乳腺细胞)
售后条例
STR位点信息
| Mouse STR 1-1 | 15 |
| Mouse STR 1-2 | 17,18 |
| Mouse STR 2-1 | 16 |
| Mouse STR 3-2 | 14 |
| Mouse STR 4-2 | 21.3 |
| Mouse STR 5-5 | 13,15 |
| Mouse STR 6-4 | 18 |
| Mouse STR 6-7 | 12 |
| Mouse STR 7-1 | 25.2 |
| Mouse STR 8-1 | 13 |
| Mouse STR 11-2 | 18 |
| Mouse STR 12-1 | 16 |
| Mouse STR 13-1 | 16.2 |
| Mouse STR 15-3 | 22.3,23.3 |
| Mouse STR 17-2 | 16 |
| Mouse STR 18-3 | 18,19 |
| Mouse STR 19-2 | 13 |
| Mouse STR X-1 | 24 |
STR鉴定图


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