Caco-2(人结直肠腺癌细胞)
¥1200.00
CaKi-1(人肾透明细胞癌皮肤转移细胞)
活动价：
¥880.00
市场价：¥1800.00
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产品规格：
- 1×10^6Cells/T25 (常温)
- 1×10^6Cells/Vial (冻存)
货号：
CL0344
培养条件：
McCoy's 5A+10%FBS+1%P/S
培养基：
- 细胞信息
- 注意事项
- STR鉴定
细胞信息
产品名称
CaKi-1
形态
上皮细胞样
冻存条件
90%FBS+10%DMSO
别名
CAKI-1; CaKi-1; caki-1; CAKI.1; CAKI 1; CAKI1; Caki1
培养条件
McCoy's 5A+10%FBS+1%P/S
生长特性
贴壁
换液频率
每周2~3次
生物安全等级
BSL-1
细胞背景
种属
人源
组织来源
肾
肿瘤类型
透明细胞癌
供体信息
49岁白人男性
背景描述
Caki-1 细胞系来源于人肾透明细胞癌的转移部位。Caki-1 细胞由位于男性患者肾静脉壁的肿瘤建立而成，通常用于肾癌生物学研究，特别是在了解透明细胞肾细胞癌 （ccRCC） 的潜在机制方面。该细胞系在形态上呈上皮样，并表现出强大的体外生长特性，使其适用于各种实验技术，包括药物筛选和分子生物学研究。
遗传背景
致瘤性
Yes, in nude mice
抗原表达
Blood Type O; Rh-; HLA A9, B12, Bw35
核型
modal number = 68; range = 63 to 71.The cell line is aneuploid human, with chromosome counts in the triploid range. The Y chromosome is absent; however, loss of the Y chromosome is not unusual from male tumor cell lines. All normal autosomes except chromosome N9 and N19 are represented, usually by two or three copies. Chromosome N9 is recognized as a marker chromosome (M1) that is usually trisomic. Normal chromosome N5 is and chromosomes N10 and N16 tend to be over-represented with respect to the copy number of other normal chromosomes. Thirteen marker chromosomes are identified: 9q+, t(1p;?), t(1qter>1q21::20), t(1q17q), der(11)t(3;11)(q21;q14), 19q+, 4q+, 4p+ and others. The chromosome counts and general cytogenetic features are in keeping with those described by J. Fogh, et al., J. Natl. Cancer Inst. (Bethesda) 58: 209, 1977.
注意事项
- 该细胞为贴壁细胞，正常传代培养选用胰酶消化，消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响，消化过程中应该注意培养细胞形态的变化，一旦胞质回缩，连接变松散，或有成片浮起的迹象就要立即终止消化，同时注意传代应在细胞处于对数期、未达到汇合状态时进行。
细胞操作
-
CaKi-1(人肾透明细胞癌皮肤转移细胞)
复苏或冻存细胞到货处理方式 -
CaKi-1(人肾透明细胞癌皮肤转移细胞)
培养操作步骤 -
CaKi-1(人肾透明细胞癌皮肤转移细胞)
售后条例
STR位点信息
| Amelogenin | X |
| CSF1PO | 10,11 |
| D2S1338 | 17 (PubMed=19372543) 17,19 (ATCC=HTB-46; CCRID; DSMZ=ACC-731) |
| D3S1358 | 17 |
| D5S818 | 11,12 |
| D7S820 | 8,12 (ATCC=HTB-46; CCRID; CLS=300149; Cosmic-CLP=905963; DSMZ=ACC-731; JCRB=JCRB0801; KCLB=30046; PubMed=25877200; RCB=RCB1985) 12 (PubMed=19372543) |
| D8S1179 | 12,14 |
| D13S317 | 11 (DSMZ=ACC-731) 11,12 (ATCC=HTB-46; CCRID; CLS=300149; Cosmic-CLP=905963; JCRB=JCRB0801; KCLB=30046; PubMed=19372543; PubMed=25877200; RCB=RCB1985) |
| D16S539 | 12 |
| D18S51 | 14 |
| D19S433 | 14 |
| D21S11 | 28,30 |
| FGA | 26 |
| Penta D | 11,12 |
| Penta E | 11,22,23 (ATCC=HTB-46) 22,23 (CLS=300149; DSMZ=ACC-731; PubMed=25877200) |
| TH01 | 6,8 |
| TPOX | 8,11 |
| vWA | 15,17 |
STR鉴定图
