作者：Zi Li,Jia-Ming Tian,Yi Chu,Hong-Yi Zhu,Jun-Jie Wang,Jun Huang
单位：中南大学湘雅二医院
期刊：Journal of Bone Oncology
产品：U2OS(人骨肉瘤细胞)
U2OS(人骨肉瘤细胞)
活动价：
¥880.00
市场价：¥1800.00
一键复制产品信息
产品规格：
- 1×10^6Cells/T25 (常温)
- 1×10^6Cells/Vial (冻存)
货号：
CL0326
培养条件：
McCoy's 5A+10%FBS+1%P/S
培养基：
- 细胞信息
- 注意事项
- STR鉴定
- 引用文献
细胞信息
产品名称
U2OS
形态
上皮细胞样
冻存条件
90%FBS+10%DMSO
别名
U-2 OS; U-2OS; U-2-OS; U2-OS; U20-S; U20S; 2T
培养条件
McCoy's 5A+10%FBS+1%P/S
生长特性
贴壁
换液频率
每周2~3次
生物安全等级
BSL-1
细胞背景
种属
人源
组织来源
胫骨
肿瘤类型
骨 肉瘤
供体信息
15岁白人女性
背景描述
U2OS 细胞是一种源自人类骨肉瘤患者的骨肉瘤细胞系，在癌症研究中发挥着重要作用，尤其是在骨癌研究中。U2OS 细胞广泛用于癌症研究、药物开发、细胞凋亡研究、遗传研究和放射肿瘤学研究。U2OS细胞的价值在于它们用于研究细胞凋亡和耐药性，这对于制造小分子抑制剂和类似治疗剂至关重要。
遗传背景
蛋白表达
Osteosarcoma derived growth factor (ODGF)
受体表达
Insulin-like growth factor I (IGF-I), insulin-like growth factor II (IGF-I), Osteosarcoma derived growth factor (ODGF)
同工酶
PGM3, 1, PGM1, 2, ES-D, 1, AK-1, 1, GLO-1, 2, G6PD, B, Phenotype Frequency Product: 0.0082
抗原表达
Blood Type A, Rh+, HLA A2, Aw30, B12, Bw35, B40(+/-)
核型
Cell line U-2 OS is chromosomally highly altered, with chromosome counts in the hypertriploid range. We did not find the hypodiploid cell population described by J. Ponten, et al., Instead, most of the population has slightly higher counts than first described. Very few normal chromosomes are present, but a high number of stable marker chromosomes are identified., Different chromosomal rearrangements involving the same chromosomes (N1, N7, N9, and N11 particularly), are seen. Twenty-two markers are found including: t(9qter--->9q21::1p36--->1p::?), 7p+, iso(17q), t(15q;?), 4q+, del(3)(q21), 5q(aberrant) and others.
注意事项
- 该细胞为贴壁细胞，正常传代培养选用胰酶消化，消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响，消化过程中应该注意培养细胞形态的变化，一旦胞质回缩，连接变松散，或有成片浮起的迹象就要立即终止消化，同时注意传代应在细胞处于对数期、未达到汇合状态时进行。
细胞操作
-
U2OS(人骨肉瘤细胞)
复苏或冻存细胞到货处理方式 -
U2OS(人骨肉瘤细胞)
培养操作步骤 -
U2OS(人骨肉瘤细胞)
售后条例
STR位点信息
| Amelogenin | X |
| CSF1PO | 12,13 (CLS=300364; DSMZ=ACC-785; Technion Genomics Center) 13 (ATCC=HTB-96; CCRID; Cosmic-CLP=909776; ECACC=92022711; KCLB=30096; PubMed=25877200) |
| D1S1656 | 16,17.3 |
| D2S441 | 10,14 |
| D2S1338 | 20,24 |
| D3S1358 | 16 |
| D5S818 | 8,11 (CLS=300364; DSMZ=ACC-785; Technion Genomics Center) 11 (ATCC=HTB-96; CCRID; Cosmic-CLP=909776; ECACC=92022711; KCLB=30096; PubMed=19787792; PubMed=25877200) |
| D6S1043 | 11 |
| D7S820 | 11,12 (ATCC=HTB-96; CCRID; CLS=300364; Cosmic-CLP=909776; DSMZ=ACC-785; ECACC=92022711; KCLB=30096; Technion Genomics Center) 12 (PubMed=25877200) |
| D8S1179 | 12,14 |
| D10S1248 | 13,14 |
| D12S391 | 19,20 |
| D13S317 | 13 |
| D16S539 | 11,12 |
| D18S51 | 12,14 (CLS=300364; DSMZ=ACC-785; Technion Genomics Center) 14 (ATCC=HTB-96; CCRID; PubMed=19787792; PubMed=25877200) |
| D19S433 | 15 |
| D21S11 | 31 |
| D22S1045 | 15,16 |
| FGA | 20 |
| Penta D | 9 |
| Penta E | 10,13 (ATCC=HTB-96; PubMed=25877200; Technion Genomics Center) 13 (DSMZ=ACC-785) |
| SE33 | 19 |
| TH01 | 6,9.3 |
| TPOX | 11,12 |
| vWA | 14,18 |
STR鉴定图
8.10
IF
作者：Shun-Hong Luo,Jia-Ming Tian,Yi Chu,Hong-Yi Zhu,Jiang-Dong Ni,Jun Huang
单位：中南大学湘雅二医院
期刊：Cell Death & Disease
