磷酸盐缓冲液（PBS）1X
¥48.00
T98G(人胶质母细胞瘤)
活动价：
¥880.00
市场价：¥1200.00
一键复制产品信息
- 细胞信息
- 注意事项
- STR鉴定
细胞信息
产品名称
T98G
形态
成纤维细胞样
冻存条件
90%FBS+10%DMSO
别名
T 98 G; T-98G; T98 G; T98-G
培养条件
MEM+10%FBS+1%P/S
生长特性
贴壁
换液频率
每周2~3次
生物安全等级
BSL-1
细胞背景
种属
人源
组织来源
脑
肿瘤类型
胶质母细胞瘤
供体信息
61岁男性欧洲人
背景描述
T98G [T98-G] 是一种成纤维细胞样细胞，是从多形性胶质母细胞瘤 White 的大脑中分离出来的
遗传背景
核型
This is a human cell line with hyperpentaploid chromosome count. The modal chromosome number should be around 128 to 132. The rate of cells with higher ploidies was 1.39%. Fourteen to 16 marker chromosomes were common to most cells. They were: der(1)t(1;?) (p36;?), i(6p), der(10)t(10;?) (q24;?), der (19)t(19;?) (q13;?), der(15)t(15;?) (q26?;?), minute metacentric and eight to ten others. Most of these structurally altered markers had complex interchromosomal exchanges. The der(10) and der(19) could be formed from a balanced translocation, i.e., t(10;19) (q24;q13). These two markers and the minute metacentric were present in three or more copies in most cells. There were six or more copies for N5, N7, N11, N13, N20, N21, and N22 in most cells. The X and N15 had only one copy.
注意事项
- 该细胞为贴壁细胞，正常传代培养选用胰酶消化，消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响，消化过程中应该注意培养细胞形态的变化，一旦胞质回缩，连接变松散，或有成片浮起的迹象就要立即终止消化，同时注意传代应在细胞处于对数期、未达到汇合状态时进行。
细胞操作
-
T98G(人胶质母细胞瘤)
复苏或冻存细胞到货处理方式 -
T98G(人胶质母细胞瘤)
培养操作步骤 -
T98G(人胶质母细胞瘤)
售后条例
STR位点信息
| Amelogenin | X,Y |
| CSF1PO | 10,12 |
| D2S1338 | 19,24 |
| D3S1358 | 16 |
| D5S818 | 10,12 |
| D7S820 | 9,10 |
| D8S1179 | 13,14 |
| D13S317 | 13 |
| D16S539 | 13 |
| D18S51 | 13,16 |
| D19S433 | 12,13 |
| D21S11 | 28,32.2 |
| FGA | 21 (PubMed=22570425; PubMed=25877200) 21,22 (ATCC=CRL-1690; COG; KCLB=21690) |
| Penta D | 10,11 |
| Penta E | 16 |
| TH01 | 7,9.3 |
| TPOX | 8 |
| vWA | 17,20 |
STR鉴定图
细胞培养最优解
套餐
组合价
组合价
¥1080
¥1568
