作者：Guanghua Chen, Jia Yan, Zhou Fu
单位：重庆市儿童医院
期刊：International Journal of Genomics
SK-MEL-28(人皮肤恶性黑色素瘤细胞)
活动价：
¥880.00
市场价：¥1200.00
一键复制产品信息
产品规格：
- 1×10^6Cells/T25 (常温)
- 1×10^6Cells/Vial (冻存)
货号：
CL0284
培养条件：
RPMI-1640+10%FBS+1%P/S
培养基：
- 细胞信息
- 注意事项
- STR鉴定
- 引用文献
细胞信息
产品名称
SK-MEL-28
形态
多角形
冻存条件
90%FBS+10%DMSO
别名
SK-Mel-28; SK.MEL.28; SK-MEL 28; SK MEL-28; SK MEL 28; SK Mel 28; SKMel-28; SKMEL-28; SK-MEL28; SK-Mel28; SK Mel28; SKMEL28; SKMel28; SKmel28; SKML-28; SK28; AU-Mel; P-36; P36
培养条件
RPMI-1640+10%FBS+1%P/S
生长特性
贴壁
换液频率
每周2~3次
生物安全等级
BSL-1
细胞背景
种属
人源
组织来源
皮肤
肿瘤类型
皮肤黑色素瘤
供体信息
51岁男性
背景描述
该细胞系是由 T.Takahashi 及其同事从一名种族不明的 51 岁男性的腋窝淋巴结中建立的，他们在一系列黑色素瘤系中分离出该细胞系。
遗传背景
蛋白表达
p53 positive
同工酶
PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1-2, GLO-1, 2, G6PD, B
致瘤性
Yes, in nude mice. Forms malignant melanoma (large round cell type)
抗原表达
Blood Type A; Rh+; HLA A11, A26, B40, DRw4
突变信息
BRAF V600E mut: V600E type BRAF Mutation was determined by DNA based methods (sequencing, RT-PCR) and protein based methods (Western Blot), N-Ras wt
核型
modal number = 90; range = 81 to 96. This is a hypotetraploid human cell line with the modal chromosome number of 90, occurring in 50% of cells. The rate of cells with a higher ploidy was 3.6%. This cell line has a very complex karyotype. There were 18 or more marker chromosomes that were common to most cells. Markers der(1)t(1;2) (p36.1;q21), del(1) (q2101) and several others had two copies per cell and t(2p14q), t(3q7p) and others had a single copy per cell. The Y/autosome translocation marker was identified as der(20)t(Y;20) (q11.23;q13.3) and had two copies per cell. The inclusion of a short segment of the euchromatic Yq11.23 was confirmed by the Southern blot DNA analysis. There were two normal X chromosomes per cell; Normal Y, N1 and N11 were absent; N19 had five or more copies per cell.
注意事项
- 该细胞为贴壁细胞，正常传代培养选用胰酶消化，消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响，消化过程中应该注意培养细胞形态的变化，一旦胞质回缩，连接变松散，或有成片浮起的迹象就要立即终止消化，同时注意传代应在细胞处于对数期、未达到汇合状态时进行。
细胞操作
-
SK-MEL-28(人皮肤恶性黑色素瘤细胞)
复苏或冻存细胞到货处理方式 -
SK-MEL-28(人皮肤恶性黑色素瘤细胞)
培养操作步骤 -
SK-MEL-28(人皮肤恶性黑色素瘤细胞)
售后条例
STR位点信息
| Amelogenin | X,Y |
| CSF1PO | 10 (PubMed=19372543; PubMed=25877200) 10,12 (AddexBio=C0020001/66; ATCC=HTB-72; CLS=300337; Cosmic-CLP=905954; PubMed=27087056; RCB=RCB1930) |
| D2S1338 | 18 |
| D3S1358 | 16,18 |
| D5S818 | 11,13 (AddexBio=C0020001/66; ATCC=HTB-72; Cosmic-CLP=905954; PubMed=27087056; RCB=RCB1930) 13 (CLS=300337; PubMed=19372543; PubMed=25877200) |
| D7S820 | 9.3,10 (AddexBio=C0020001/66; ATCC=HTB-72) 10 (CLS=300337; Cosmic-CLP=905954; PubMed=19372543; PubMed=25877200; PubMed=27087056; RCB=RCB1930) |
| D8S1179 | 13 |
| D13S317 | 11,12 |
| D16S539 | 9,12 |
| D18S51 | 12,16 |
| D19S433 | 14 |
| D21S11 | 28,29 |
| FGA | 19 |
| Penta D | 9,10 |
| Penta E | 8,12 |
| TH01 | 7 |
| TPOX | 8,12 (AddexBio=C0020001/66; ATCC=HTB-72; CLS=300337; Cosmic-CLP=905954; PubMed=27087056; RCB=RCB1930) 12 (PubMed=19372543; PubMed=25877200) |
| vWA | 16,19 (AddexBio=C0020001/66; ATCC=HTB-72; CLS=300337; Cosmic-CLP=905954; PubMed=25877200; PubMed=27087056; RCB=RCB1930) 19 (PubMed=19372543) |
STR鉴定图
细胞培养最优解
套餐
组合价
组合价
¥1080
¥1568
