SiHa(人子宫颈鳞癌细胞)

全部细胞

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活动价：

¥880.00

市场价：¥1200.00

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产品规格：

货号：

CL0280

培养条件：

DMEM+10%FBS+1%P/S

培养基：

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种属
人源
组织来源
子宫颈
肿瘤类型
人瘤病毒相关性宫颈鳞状细胞癌
供体信息
55岁亚洲女性
背景描述
SiHa细胞是一种人宫颈鳞状细胞癌细胞系，几十年来已广泛用于研究。它们是从一名 55 岁的日本女性鳞状细胞癌患者的原发性子宫活检片段中分离出来的。由于其独特的遗传特征，该细胞系对研究宫颈癌和其他相关疾病的研究人员非常感兴趣。

同工酶
AK-1,1；ES-D, 2；G6PD, B；GLO-I, 2；Me-2, 1；PGM1, 1；PGM3, 1
基因表达
p53+; pRB+
致瘤性
Yes, in nude mice; forms poorly differentiated epidermoid carcinoma (grade III)
核型
modal number = 69; range = 51 to 72.This is a hypertriploid human cell line with the modal chromosome number of 71, occurring in 24% of cells. Most cells had the chromosome numbers distributed between 69 and 72. Polyploid cells occurred at 7.6%. Fifteen or more marker chromosomes were common to most cells. Among them are dup(2) (q22q31) and del(2) (q31) which probably resulted from the balanced translocation between two N2s. Most cells had two copies of del(2). M2 is an A3-sized acrocentric. M13 is a minute submetacentric with 1-3 copies per cell. Origins of both M2 and M13 are not identified. There were two copies of normal X chromosomes. N2 was absent and probably was replaced by dup(2) and del(2).

该细胞为贴壁细胞，正常传代培养选用胰酶消化，消化的时间受消化液的种类、配制时间、加入培养瓶中的量等诸多因素的影响，消化过程中应该注意培养细胞形态的变化，一旦胞质回缩，连接变松散，或有成片浮起的迹象就要立即终止消化，同时注意传代应在细胞处于对数期、未达到汇合状态时进行。

Amelogenin	X
CSF1PO	12
D2S1338	24
D3S1358	16,17
D5S818	9
D7S820	10
D8S1179	13,16
D13S317	11
D16S539	8,12 (PubMed=29156801) 12 (AddexBio=C0008002/372; ATCC=HTB-35; CCRID; Cosmic-CLP=930297; KCLB=30035; PubMed=25877200)
D18S51	15
D19S433	14.2
D21S11	29,31
FGA	21
Penta D	9,12
Penta E	10,12
TH01	6,9
TPOX	8 (AddexBio=C0008002/372; ATCC=HTB-35; CCRID; Cosmic-CLP=930297; KCLB=30035; PubMed=25877200) 8,9 (PubMed=29156801)
vWA	14,17