RT-4(人膀胱移行细胞乳头瘤细胞)
活动价:
市场价:¥2000.00
- 1×10^6Cells/T25 (常温)
- 1×10^6Cells/Vial (冻存)
CL0269
McCOY's 5A+10%FBS+1%P/S
- 细胞信息
- 注意事项
- STR鉴定
细胞信息
产品名称
RT-4
形态
上皮细胞样
冻存条件
90%FBS+10%DMSO
别名
RT4; RT4P
培养条件
McCOY's 5A+10%FBS+1%P/S
生长特性
贴壁
换液频率
每周2~3次
生物安全等级
BSL-1
细胞背景
种属
人源
组织来源
膀胱
肿瘤类型
移行细胞状瘤
供体信息
63岁白人男性
背景描述
1968 年,一名 63 岁男性的复发性高分化移行性膀胱移行状肿瘤(临床分期 T2,组织学分级 G1,2 年前用金颗粒治疗,10 个月后复发后透热治疗)
遗传背景
蛋白表达
p53 positive
同工酶
AK-1, 1;ES-D, 1-2;G6PD, B;GLO-I, 1-2;Me-2, 1;PGM1, 1-2;PGM3, 1-2
致瘤性
Yes, in cheek pouch of steroid-treated hamsters
抗原表达
HLA A25(10), A3, B12, Cw3; Blood Type O
核型
modal number = 49; range = 42 to 55. The cell line is aneuploid human male, with chromosome counts in the near-diploid range. However, the near-tetraploid population may become predominant within very few passages. The 48 and 49 chromosome karyotypes had a single X and a single Y chromosome. Three of the karyotypes at higher ploidy had two X chromosomes and a single Y chromosome. Four marker chromosomes were identified: del(6)(q21), del(10)(p11), 14p+, 12p+. Marker chromosome M4 was not seen in the karyotypes prepared from higher ploidy metaphases.
注意事项
- 4.细胞对血清要求比较高,建议使用来源进口优质血清;
细胞操作
-
RT-4(人膀胱移行细胞乳头瘤细胞)
复苏或冻存细胞到货处理方式 -
RT-4(人膀胱移行细胞乳头瘤细胞)
培养操作步骤 -
RT-4(人膀胱移行细胞乳头瘤细胞)
售后条例
STR位点信息
| Amelogenin | X,Y |
| CSF1PO | 10,12 |
| D2S1338 | 18,19 |
| D3S1358 | 15 |
| D5S818 | 11,12 |
| D7S820 | 9,12 |
| D8S1179 | 13,15 |
| D13S317 | 8 |
| D16S539 | 9 |
| D18S51 | 15,17 |
| D19S433 | 13 |
| D21S11 | 30,32.2 |
| FGA | 22,24 |
| Penta D | 12 (ATCC=HTB-2; CLS=300326; DSMZ=ACC-412) 12,13 (PubMed=25877200) |
| Penta E | 7,10 |
| TH01 | 9,9.3 |
| TPOX | 8,11 |
| vWA | 14,17 |
STR鉴定图














