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CL0268
IMDM+15%FBS+1%P/S
RPMI-8226
淋巴母细胞样
95%FBS+5%DMSO
RPMI 8226; RPMI.8226; RPMI8226; RPMI no. 8226; RPMI no 8226; RPMI #8226; 8226; RPMI 8226/S; RPMI-8226S; RPMI8226/S; 8226/S; Roswell Park Memorial Institute 8226; GM02132; GM2132; GM 2132; GM02132C; Simpson
IMDM+15%FBS+1%P/S
悬浮
每周2~3次
BSL-1
人源
外周血
浆细胞瘤
61岁男性
RPMI 8226 是 1966 年从一名 61 岁男性浆细胞瘤患者的外周血中分离出的 B 淋巴细胞。该细胞系以其开发的罗斯威尔公园纪念研究所 （RPMI） 命名，数字 8226 表示其在细胞库中的特定目录号。 RPMI 8226 细胞系是研究多发性骨髓瘤以及浆细胞生物学、免疫学研究和癌症治疗相关方面的重要模型系统。已知 RPMI 8226 细胞产生和分泌免疫球蛋白的 κ 轻链，这一特征在研究中经常被用于研究抗体的产生和分泌机制。
G6PD, A
immunoglobulin light chain
HLA Aw19, B15, B37, Cw2; CD19 -; CD20 -; CD28 +; CD38 +; CD49e +
Human poliovirus 1，Herpes simplex virus，Vaccinia virus，Vesicular stomatitis virus
阴性
human flat-moded hypotriploid karyotype with 7.5% polyploidy - 62-67<3n>XXY, -1, +5, +6, +7, -8, -9, -10, +11, -12, -13, -14, +16, +18, -19, +21, -22, der(1)t(1;1)(p36.3;q11-12), add(3)(q27-29)x2, del(4)(p14p15.4), der(5;6)(q10;p10)x1-2, del(5)(q32), del(6)(q15), add(9)(p24), add(11)(p15), del(11)(q25), der(14)t(1;14)(q11-12;q32)x2, del(15)(q11.2q14)x2, add(16)(q23-24), der(17)t(?8;17)(q21.2;q25), del(22)(q13.2) - der(1), 6q-, 14q+ and 22q- associated with multiple myeloma - identified by disomic 14q+，Two large marker chromosomes with terminal centromeres.
RPMI-8226(人多发性骨髓瘤细胞)
复苏或冻存细胞到货处理方式RPMI-8226(人多发性骨髓瘤细胞)
培养操作步骤RPMI-8226(人多发性骨髓瘤细胞)
售后条例| Amelogenin | X (PubMed=19372543; TKG=TKG 0243) X,Y (AddexBio=C0003002/62; ATCC=CCL-155; CCRID; CLS=300431; Cosmic-CLP=905964; DSMZ=ACC-402; ECACC=87012702; Technion Genomics Center BCF; JCRB=IFO50013; JCRB=JCRB0034; KCLB=10155; PubMed=25877200) |
| CSF1PO | 12 |
| D1S1656 | 13 |
| D2S441 | 12,14 |
| D2S1338 | 20 |
| D3S1358 | 16,17 |
| D5S818 | 11,13 (AddexBio=C0003002/62; ATCC=CCL-155; CCRID; CLS=300431; Cosmic-CLP=905964; DSMZ=ACC-402; ECACC=87012702; Technion Genomics Center BCF; JCRB=IFO50013; JCRB=JCRB0034; KCLB=10155; PubMed=19372543; TKG=TKG 0243) 13 (PubMed=25877200) |
| D7S820 | 9,10 |
| D8S1179 | 12,13 (ATCC=CCL-155; CCRID) 13 (CLS=300431; DSMZ=ACC-402; Technion Genomics Center BCF; PubMed=25877200) |
| D10S1248 | 12 |
| D12S391 | 16,19 |
| D13S317 | 11 |
| D16S539 | 9 |
| D18S51 | 15,19 |
| D19S433 | 13.2,14 (ATCC=CCL-155; CCRID; Technion Genomics Center BCF; PubMed=19372543) 14 (DSMZ=ACC-402) |
| D21S11 | 28,29 |
| D22S1045 | 15,17 |
| DYS391 | 11 |
| FGA | 19 |
| Penta D | 2.2,11 (ATCC=CCL-155; CLS=300431; Technion Genomics Center BCF; PubMed=25877200) 11 (DSMZ=ACC-402) |
| Penta E | 16,17 |
| TH01 | 8 |
| TPOX | 8,11 |
| vWA | 16 (DSMZ=ACC-402) 16,18 (AddexBio=C0003002/62; ATCC=CCL-155; CCRID; CLS=300431; Cosmic-CLP=905964; ECACC=87012702; Technion Genomics Center BCF; JCRB=IFO50013; JCRB=JCRB0034; KCLB=10155; PubMed=19372543; PubMed=25877200; TKG=TKG 0243) |
IF
作者：Bingxin Zhang,Quanqiang Wang,Zhili Lin,Ziwei Zheng,Shujuan Zhou,Tianyu Zhang,Dong Zheng,Zixing Chen,Sisi Zheng,Yu Zhang,Xuanru Lin,Rujiao Dong,Jingjing Chen,Honglan Qian,Xudong Hu,Yan Zhuang,Qianying Zhang,Zhouxiang Jin,Songfu Jiang,Yongyong Ma
单位：温州医科大学附属第一医院
期刊：Frontiers in cell and developmental biology
DOI：10.3389/fcell.2023.1198949
产品：RPMI-8226(人多发性骨髓瘤细胞)、NCI-H929(人骨髓瘤细胞)、MM1.R(人多发性骨髓瘤)、U266(人骨髓瘤细胞)
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