MOLT-4(人急性淋巴母细胞性白血病细胞)

活动价:

¥1200.00

市场价:¥1440.00

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产品规格:
  • 1×10^6Cells/T25 (常温)
  • 1×10^6Cells/Vial (冻存)
货号:

CL0224

培养条件:

RPMI-1640+10%FBS+1%P/S

  • 细胞信息
  • 注意事项
  • STR鉴定
  • 引用文献
  • 实验验证
  • 相关产品
  • 文件下载

细胞信息

  • 产品名称

    MOLT-4

  • 形态

    淋巴母细胞样

  • 冻存条件

    95%FBS+5%DMSO

  • 别名

    Molt-4;MOLT4;Molt4;MOLT.4;MOLT4;Molt4;GM02219;GM02219C;GM2219C;GM02219D

  • 培养条件

    RPMI-1640+10%FBS+1%P/S

  • 生长特性

    悬浮

  • 换液频率

    每周2~3次

  • 生物安全等级

    BSL-1

细胞背景

  • 种属

    人源

  • 组织来源

    外周血

  • 肿瘤类型

    成人 T 急性淋巴细胞白血病

  • 供体信息

    19岁白人男性

  • 背景描述

    MOLT-4是一种T淋巴细胞系,来源于1971年复发的一名19岁急性淋巴细胞白血病(ALL)男性患者的外周血。它是MOLT-3的姊妹细胞系,而MOLT-4显示出不寻常的T细胞抗原受体γ链基因(T-γ)重排。MOLT-4细胞的倍增时间约为30小时,悬浮生长,并且在未经治疗的裸鼠、抗淋巴细胞血清处理的小鼠和X辐照小鼠中具有致瘤性。

遗传背景

  • 蛋白表达

    p53 positive

  • 基因表达

    High levels of terminal deoxynucleotidyl transferase (TdT) are produced

  • 致瘤性

    Yes, in untreated nude mice, anti lymphocyte serum treated mice and X-irradiated mice

  • 抗原表达

    CD1 (49%), CD2 (35%), CD3 A (26%) B (33%) C (34%), CD4 (55%), CD5 (72%), CD6 (22%), CD7 (77%)

  • 病毒

    The cells do not produce immunoglobulin or Epstein-Barr virus (Minowada, 1972).

  • 突变信息

    G -> A mutation at codon 248 of the p53 gene, P53 is not expressed (Rodrigues, 1990).

  • 核型

    Hypertetraploid. Modal number: 96. Two x and two Y chromosomes.

注意事项

  • 该细胞为悬浮细胞,在培养的过程中可以通过补加培养基、半量换液或者全换液的方式补充营养物质。在培养过程中若细胞增殖速度较慢或细胞状态不佳,需尽量减少操作频次,保种细胞稳定生长的环境。细胞培养过程中需要较好的控制细胞密度,一般在2×10^5~1×10^6个/ml。

细胞操作

STR位点信息

Amelogenin X,Y
CSF1PO 11,12 (CLS=300115; DSMZ=ACC-362) 11,12,13 (ATCC=CRL-1582; CCRID; COG; Cosmic-CLP=905958; JCRB=IFO50362; JCRB=JCRB9031; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763; PubMed=25877200)
D2S1338 23,24
D3S1358 14,16 (ATCC=CRL-1582; CCRID; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763; PubMed=25877200) 15,16 (CLS=300115; DSMZ=ACC-362)
D5S818 11,12 (DSMZ=ACC-362) 12 (ATCC=CRL-1582; CCRID; CLS=300115; COG; Cosmic-CLP=905958; JCRB=IFO50362; JCRB=JCRB9031; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763) 12,13 (PubMed=25877200)
D7S820 8,10 (DSMZ=ACC-362) 8,10,11 (ATCC=CRL-1582; CCRID; CLS=300115; COG; Cosmic-CLP=905958; JCRB=IFO50362; JCRB=JCRB9031; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763; PubMed=25877200)
D8S1179 9,13,14 (ATCC=CRL-1582; CLS=300115; DSMZ=ACC-362) 9,13,14,15 (PubMed=25877200) 9,14 (CCRID; COG; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763) 9,14,15 (PubMed=11416159)
D13S317 12,13 (ATCC=CRL-1582; CCRID; CLS=300115; COG; Cosmic-CLP=905958; JCRB=IFO50362; JCRB=JCRB9031; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763; PubMed=25877200) 12,13,14 (DSMZ=ACC-362)
D16S539 11,14 (ATCC=CRL-1582; CCRID; CLS=300115; Cosmic-CLP=905958; DSMZ=ACC-362; JCRB=IFO50362; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763) 11,14,15 (COG; JCRB=JCRB9031; PubMed=25877200)
D18S51 12,13,16,17,18 (DSMZ=ACC-362) 12,13,17 (CLS=300115) 13,16,17 (ATCC=CRL-1582; DOI=10.4172/2157-7145.S2-005) 13,17 (CCRID; PubMed=19372543; PubMed=20922763; PubMed=25877200)
D19S433 14,15 (ATCC=CRL-1582; CCRID; DOI=10.4172/2157-7145.S2-005; DSMZ=ACC-362; PubMed=20922763; PubMed=25877200) 14,15,16 (PubMed=19372543)
D21S11 27,28,29,30 (ATCC=CRL-1582) 27,28,29,30,31 (COG; DOI=10.4172/2157-7145.S2-005; PubMed=25877200) 28,29,30 (CLS=300115; DSMZ=ACC-362; PubMed=11416159) 28,29,30,31 (CCRID; PubMed=19372543; PubMed=20922763)
FGA 21,22,23,24,25 (PubMed=25877200) 21,22,24,25 (DSMZ=ACC-362) 22,23,24,25 (ATCC=CRL-1582; COG; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763) 22,23,25 (CCRID) 22,24 (CLS=300115) 22,25 (PubMed=11416159)
Penta D 8,12,13 (CLS=300115; DSMZ=ACC-362) 8,13 (ATCC=CRL-1582; PubMed=25877200)
Penta E 14,15 (DSMZ=ACC-362) 14,15,16 (CLS=300115) 14,16 (ATCC=CRL-1582; PubMed=25877200)
TH01 6,8
TPOX 8
vWA 16,17,18 (DSMZ=ACC-362) 17 (PubMed=11416159) 17,18 (ATCC=CRL-1582; CCRID; CLS=300115; COG; Cosmic-CLP=905958; JCRB=IFO50362; JCRB=JCRB9031; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=20922763; PubMed=25877200) 17,18,19 (PubMed=19372543)

STR鉴定图