铜绿假单胞菌
¥700.00
- 菌株信息
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产品名称
深红红螺菌
拉丁名称
Rhodospirillumrubrum
模式菌株
是
参考用途
科研
生物危害程度
四类
培养温度
30℃
需氧类型
厌氧
别名
DSM467=ATCC11170=NCIB8255
培养条件
红螺菌培养基酵母提取物 0.30 g 乙醇 0.50 ml 琥珀酸钠 1.00 g 乙酸铵 0.50 g 柠檬酸铁(0.1% in H2O) 5.00 ml KH2PO4 0.50 g MgSO4x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g 维生素B12 组份(10 mg in 100 ml H2O) 0.40 ml 微量元素组份 SL-6 (如下) 1.00 ml 蒸馏水 1050.00 ml pH 6.8. Boil the medium under a stream of nitrogen gas for few minutes and distribute 45 ml medium into 50 ml screw-capped bottles (already flushed with nitrogen gas). Bubble each bottle with nitrogen gas and close immediately with a rubber septum and screw tight. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp. For brown and other oxygen sensitive Rhodospirillaceae add 300 mg of L-cysteine (0.03% end concentration) to the boiling medium and readjust the pH to 6.8 or to the prepared medium in bottles inject neutralized sulfide solution (0.005 to 0.01% end concentration). The medium has been modified according to reference 3365. 微量元素组份SL-6: ZnSO4 x 7 H2O 0.10 g MnCl2 x 4 H2O 0.03 g H3BO3 0.30 g CoCl2 x 6 H2O 0.20 g CuCl2 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.02 g Na2MoO4 x 2 H2O 0.03 g 蒸馏水 1000.00 ml
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