铜绿假单胞菌
¥700.00
栖酒窖共养单胞菌
活动价：
¥1680.00
市场价：¥1880.00
一键复制产品信息
货号：
FHJZ74314
拉丁学名：
Syntrophomonas cellicola
培养温度：
37℃
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产品名称
栖酒窖共养单胞菌
拉丁名称
Syntrophomonas cellicola
模式菌株
是
参考用途
模式菌株
生物危害程度
四类
培养温度
37℃
保存温度
冷藏4-10℃
别名
=JCM 13582
培养条件
1064Solution A:Trypticase peptone (BD BBL)1.0g Mineral solution50.0mlTrace element solution SL-101.0mlRumen fluid clarified50.0mlButyric acid1.7gNa2SO42.8 gNa-resazurin solution (0.1% w/v)0.5ml蒸馏水 830.0ml Solution B:NaHCO3 3.5g蒸馏水50.0mlSolution C:Vitamin solution1.0mlSolution D:L-Cysteine-HCl·H2O0.3g蒸馏水10.0mlSolution E:Na2S·9H2O0.3g蒸馏水10.0mlAdd and dissolve ingredients of solution AMineral solution：KH2PO410.0gMgCl2·6 H2O6.6gNaCl8.0g NH4Cl8.0gCaCl2·2 H2O1.0g蒸馏水1.0LTrace element solution SL-10：HCl (25%; 7.7 M) 10.0ml FeCl2·4 H2O1.5gZnCl270.0mgMnCl2·4 H2O100.0mgH3BO36.0mgCoCl2·6 H2O190.0mgCuCl2 ·2 H2O2.0mgNiCl2·6H2O24.0mgNa2MoO4·2H2O36.0mg蒸馏水990.0mlFirst dissolve FeCl2 in the HCl then dilute in water add and dissolve the other salts. Finally make up to 1000.0 ml.Clarified rumen fluid:Rumen fluid from cow or sheep is filtered through muslin autoclaved at 121°C for 15 min and then centrifuged at 27000g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C.Seven vitamins solution：Vitamin B12100.0mgp-Aminobenzoic acid80.0mgD(+)-Biotin20.0mgNicotinic acid200.0mgCalcium pantothenate100.0mgPyridoxine hydrochloride300.0mgThiamine-HCl x· 2 H2O200.0mg蒸馏水1.0L
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