铜绿假单胞菌
¥700.00
- 菌株信息
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产品名称
栖温泉螺旋体
拉丁名称
Treponema caldarium
模式菌株
是
参考用途
模式菌株
生物危害程度
四类
培养温度
50℃
保存温度
冷藏4-10℃
别名
=ATCC 51460
培养条件
1062D-Glucose2.0gTryptone2.0gNa-resazurin solution (0.1% w/v)0.5mlL-Cysteine-HCl·H2O0.25gTrace element solution SL-1010.0mlTrace element solution（见培养基0940）25.0mlVitamin solution（见培养基0901）10.0ml蒸馏水 1000.0ml Dissolve ingredients (except cysteine trace elements and vitamins) sparge medium with 100% N2 gas for 30 – 45 min to make it anoxic. Then add cysteine adjust pH to 7.0 dispense medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add trace elements and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Check pH of complete medium and adjust to 7.0 if necessary.Trace element solution SL-10：HCl (25%; 7.7 M) 10.0ml FeCl2·4 H2O1.5gZnCl270.0mgMnCl2·4 H2O100.0mgH3BO36.0mgCoCl2·6 H2O190.0mgCuCl2 ·2 H2O2.0mgNiCl2·6H2O24.0mgNa2MoO4·2H2O36.0mg蒸馏水990.0mlFirst dissolve FeCl2 in the HCl then dilute in water add and dissolve the other salts. Finally make up to 1000.0 ml.
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