腾冲热互养菌

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¥1680.00

市场价:¥1880.00

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货号:

FHJZ74274

拉丁学名:

Thermosyntropha tengcongensis

培养温度:

60℃

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  • 产品名称

    腾冲热互养菌

  • 拉丁名称

    Thermosyntropha tengcongensis

  • 模式菌株

    是

  • 参考用途

    模式菌株

  • 生物危害程度

    四类

  • 培养温度

    60℃

  • 保存温度

    冷藏4-10℃

  • 别名

    =JCM 17260

  • 培养条件

    THERMOSYNTROPHA L-60 MEDIUM0977 THERMOSYNTROPHA L-60 MEDIUMSolution A:KH2PO4  0.54g Na2HPO4  1.33g Yeast extract (BD-Difco)  3.0g Tryptone (BD-Difco)  0.5g Bacto peptone (BD-Difco)  1.5g Resazurin  1.0mg Distilled water  760.0ml Solution B:NH4Cl  0.3g NaCl  0.3g CaCl2·2H2O  0.01g MgCl2·6H2O  0.1g Trace element solution (见培养基0901) 1.0ml Distilled water  100.0ml Solution C:Crotonic acid  1.72g Distilled water  100.0ml Adjust pH to 7.0Autoclave Solutions A B and C separately. After cooling aseptically and anaerobically combine the three solutions under a stream of N2-CO2 (80:20 v/v) then add the following solutions (filter-sterilized):Trace vitamins见培养基0901 10.0ml 8% NaHCO3 solution  30.0ml Prior to inoculation reduce the medium with 0.025% L-cysteine·HCl·H2O and 0.025% Na2S·9H2O (use 5% solutions autoclaved and stored under a N2 atmosphere).

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