阳极还原地杆菌

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活动价：

¥1680.00

市场价：¥1880.00

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货号：

FHJZ73020

拉丁学名：

Geobacter anodireducens

培养温度：

30℃

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产品名称
阳极还原地杆菌
拉丁名称
Geobacter anodireducens
模式菌株
是
参考用途
模式菌株
生物危害程度
四类
培养温度
30℃
保存温度
冷藏4-10℃
别名
=KCTC 4672
培养条件
1055sodium acetate0.82gCaCl2·2H2O0.147gNH4Cl0.535g NaH2PO40.136gNa2SO40.142gTrace element solution10.0mlVitamins solution（见培养基0901）10.0ml Se/W solution（见培养基0975）1.0mlDistilled water 1.0L Mix components thoroughly and autoclave under a N2-CO2 (80:20 v/v) gas mixture. After cooling add aseptically and anaerobically the following solutions to the medium：8% NaHCO3 solution (filter-sterilized) 30.0ml 100 mM Fe NTA solution (see below) 50.0ml Adjust pH to 7.0 - 7.2100 mM Fe NTA solution:NaHCO3 1.64gNitrilotriacetic acid trisodium salt monohydrate 2.75gFeCl3·6H2O 2.7gFirstly dissolve NaHCO3 in 80 ml distilled water. Then add NTA and FeCl3·6H2O to the solution and bring volume to 100 ml with distilled water. Adjust pH to 7.0 with NaOH. Filter-sterilize and store under a N2 atmosphereTrace element solution：Nitrilotriacetic acid 12.8g FeCl3·6H2O 1.35g MnCl2·4H2O 0.1g CoCl2·6H2O 0.024g CaCl2·2H2O 0.1g ZnCl2 0.1g CuCl2·2H2O 0.025g H3BO3 0.01g NaCl 1.0g NiCl2·6H2O 0.12g Na2SeO3·5H2O 0.026g Distilled water 1.0L Dissolve nitrilotriacetic acid first in 200 ml of distilled water and adjust pH to 6.5 with KOH. Then add the remaining salts and adjust volume to 1.0 L. Readjust pH to 7.0 with KOH.

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