培养条件

脂环酸芽孢杆菌培养基0437 脂环酸芽孢杆菌培养基 Solution A:CaCl2 ·2H2O0.25gMgSO4·7H2O0.5g (NH4)2 SO4 0.2gYeast extract2.0gGlucose5.0g KH2PO43.0gDistilled water (for liquid medium) 1.0L Distilled water (for solid medium)0.5LAdjust pH to 4.0Solution B:Trace element sol. SL-6 (see medium 27)1.0mlTrace element solution SL-6:ZnSO4 · 7 H2O0.1gMnCl2 · 4 H2O 0.03gH3BO3 0.3gCoCl2 · 6 H2O0.2gCuCl2 · 2 H2O0.01gNiCl2 · 6 H2O0.02gNa2MoO4 ·2 H2O0.03gDistilled water1.0LSolution C:Agar 15.0gDistilled water500.0mlSterilize separately. For liquid medium combine solution A (with 1000.0 ml distilled water) and solution B. For solid medium combine solution A (with 500 ml distilled water) solution B and solution C. For strains of A. cycloheptanicus add 5 g/l of yeast extract instead of 2 g/l.