FGF-2 Polyclonal Antibody
¥680.00
- 产品信息
商品描述
靶点-Target
MSK1
反应性
Human,Mouse,Rat,
应用
WB,IF,IP,ELISA
宿主物种-Host Species
Rabbit
同种型-Isotype
IgG,Kappa
偶联-Conjugate
Phospho
修饰-Modification
Ser360
MW(Calculated)
90kD
MW(Observed)
90kD
推荐稀释比
WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200,
组成
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
纯化工艺
Protein A
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
克隆性
Monoclonal
克隆号
PT0174R
特异性
This antibody detects endogenous levels of MSK1 only when phosphorylated at Ser360.This antibody does not recognize phosphorylated at other sites.
基因名称
RPS6KA5
蛋白名称
Ribosomal protein S6 kinase alpha-5
别名
RPS6KA5;MSK1;Ribosomal protein S6 kinase alpha-5;S6K-alpha-5;90 kDa ribosomal protein S6 kinase 5;Nuclear mitogen- and stress-activated protein kinase 1;RSK-like protein kinase;RSKL
Organism-1
Human
基因ID-1
9252
SwissProt-1
O75582
Organism-2
Mouse
基因ID-2
73086
SwissProt-2
Q8C050
背景
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Appears to be activated by multiple phosphorylations on threonine and serine residues. ERK1/2 and MAPK14/p38-alpha may play a role in this process.,function:Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).,miscellaneous:Enzyme activity requires the presence of both kinase domains.,PTM:Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 2 protein kinase domains.,subcellular location:Predominantly nuclear. Partially cytoplasmic.,subunit:Forms a complex with either ERK1 or ERK2 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA5 associates with and phosphorylates the NF-kappa-B p65 subunit RELA.,tissue specificity:Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.,
细胞定位
Cytoplasm,Nuclear
