FGF-2 Polyclonal Antibody
¥680.00
- 产品信息
商品描述
靶点-Target
Chk2
反应性
Human,Mouse,Rat
应用
WB,IHC,IF,IP,ELISA
宿主物种-Host Species
Rabbit
同种型-Isotype
IgG,Kappa
偶联-Conjugate
Phospho
修饰-Modification
Thr68
MW(Calculated)
61kD
MW(Observed)
61kD
推荐稀释比
IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;
组成
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
纯化工艺
Protein A
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
克隆性
Monoclonal
克隆号
PT0729R
特异性
Chk2 (Phospho Thr68) Antibody detects endogenous levels of Chk2 protein only when phosphorylated at T68.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):VStQE
基因名称
CHEK2
蛋白名称
Serine/threonine-protein kinase Chk2
别名
CHEK2;CDS1;CHK2;RAD53;Serine/threonine-protein kinase Chk2;CHK2 checkpoint homolog;Cds1 homolog;Hucds1;hCds1;Checkpoint kinase 2
Organism-1
Human
基因ID-1
11200
SwissProt-1
O96017
Organism-2
Mouse
基因ID-2
50883
SwissProt-2
Q9Z265
背景
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutati
细胞定位
[Isoform 2]: Nucleus. Isoform 10 is present throughout the cell.; [Isoform 4]: Nucleus.; [Isoform 7]: Nucleus.; [Isoform 9]: Nucleus.; [Isoform 12]: Nucleus.; Nucleus, PML body. Nucleus, nucleoplasm. Recruited into PML bodies together with TP53.
