FGF-2 Polyclonal Antibody
¥680.00
- 产品信息
商品描述
靶点-Target
Rock-1
反应性
Human,Mouse,Rat,Monkey
应用
IF,WB,IHC,ELISA
宿主物种-Host Species
Rabbit
同种型-Isotype
IgG
修饰-Modification
Unmodified
MW(Observed)
158kD
推荐稀释比
IF 1:50-200;WB 1:500-1:2000;IHC 1:100-1:300;ICC 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications;
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
浓度
1 mg/ml
克隆性
Polyclonal
免疫原
The antiserum was produced against synthesized peptide derived from human Rock-1. AA range:262-311
特异性
Rock-1 Polyclonal Antibody detects endogenous levels of Rock-1 protein.
基因名称
ROCK1
蛋白名称
Rho-associated protein kinase 1
别名
ROCK1;Rho-associated protein kinase 1;Renal carcinoma antigen NY-REN-35;Rho-associated;coiled-coil-containing protein kinase 1;Rho-associated, coiled-coil-containing protein kinase I;ROCK-I;p160 ROCK-1;p160ROCK
Organism-1
Human
基因ID-1
6093
SwissProt-1
Q13464
Organism-2
Mouse
基因ID-2
19877
SwissProt-2
P70335
Organism-3
Rat
基因ID-3
81762
SwissProt-3
Q63644
背景
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015],
细胞定位
Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Golgi apparatus membrane ; Peripheral membrane protein . Cell projection, bleb . Cytoplasm, cytoskeleton . Cell membrane . Cell projection, lamellipodium . Cell projection, ruffle . A small proportion is associated with Golgi membranes (PubMed:12773565). Associated with the mother centriole and an intercentriolar linker (By similarity). Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers (By similarity). Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). .
