FGF-2 Polyclonal Antibody
¥680.00
- 产品信息
商品描述
靶点-Target
PAK1/2/3
反应性
Human,Mouse,Rat
应用
IHC,IF,ELISA
宿主物种-Host Species
Rabbit
同种型-Isotype
IgG
修饰-Modification
Unmodified
MW(Calculated)
60kD
推荐稀释比
IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
浓度
1 mg/ml
克隆性
Polyclonal
免疫原
The antiserum was produced against synthesized peptide derived from human PAK1/2/3. AA range:111-160
特异性
PAKα/β/γ Polyclonal Antibody detects endogenous levels of PAKα/β/γ protein.
基因名称
PAK1/PAK2/PAK3
蛋白名称
Serine/threonine-protein kinase PAK 1
别名
PAK1;Serine/threonine-protein kinase PAK 1;Alpha-PAK;p21-activated kinase 1;PAK-1;p65-PAK;PAK2;Serine/threonine-protein kinase PAK 2;Gamma-PAK;PAK65;S6/H4 kinase;p21-activated kinase 2;PAK-2;p58;PAK3;OPHN3;Serine/threonine-p
Organism-1
Human
基因ID-1
5058;5062;5063
SwissProt-1
Q13153;Q13177;O75914
Organism-2
Mouse
基因ID-2
224105;18481
Organism-3
Rat
基因ID-3
29431;100910732;29433
SwissProt-3
P35465;Q64303;Q62829
背景
This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010],
细胞定位
Cytoplasm . Cell junction, focal adhesion . Cell projection, lamellipodium . Cell membrane . Cell projection, ruffle membrane . Cell projection, invadopodium . Nucleus, nucleoplasm . Chromosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Colocalizes with RUFY3, F-actin and other core migration components in invadopodia at the cell periphery (PubMed:25766321). Recruited to the cell membrane by interaction with CDC42 and RAC1. Recruited to focal adhesions upon activation. Colocalized with CIB1 within membrane ruffles during cell spreading upon readhesion to fibronectin. Upon DNA damage, translocates to the nucleoplasm when phosphorylated at Thr-212 where is co-recruited with MORC2 on damaged chromatin (PubMed:23260667). Localization to the centrosome does not depend upon the presence of gamma-tubulin (PubMed:27012601). Localization of the active, but not inactive, protein to the adhesions and edge of lamellipodia is mediated by interaction with GIT1 (PubMed:11896197). .
