FGF-2 Polyclonal Antibody
¥680.00
- 产品信息
商品描述
靶点-Target
MRTF-A
反应性
Human,Mouse
应用
WB,IHC,IF,ELISA
宿主物种-Host Species
Rabbit
同种型-Isotype
IgG
修饰-Modification
Unmodified
MW(Observed)
99kD
推荐稀释比
WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
浓度
1 mg/ml
克隆性
Polyclonal
免疫原
The antiserum was produced against synthesized peptide derived from human MKL1. AA range:10-59
特异性
MRTF-A Polyclonal Antibody detects endogenous levels of MRTF-A protein.
基因名称
MKL1
蛋白名称
MKL/myocardin-like protein 1
别名
MKL1;KIAA1438;MAL;MKL/myocardin-like protein 1;Megakaryoblastic leukemia 1 protein;Megakaryocytic acute leukemia protein;Myocardin-related transcription factor A;MRTF-A
Organism-1
Human
基因ID-1
57591
SwissProt-1
Q969V6
Organism-2
Mouse
基因ID-2
223701
SwissProt-2
Q8K4J6
背景
The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013],
细胞定位
Cytoplasm . Nucleus . Subcellular location is tightly regulated by actin both in cytoplasm and nucleus: high levels of G-actin in the nucleus observed during serum deprivation lead to low levels of nuclear MRTFA, while reduced levels of nuclear G-actin result in accumulation of MRTFA in the nucleus (By similarity). G-actin-binding in the cytoplasm inhibits nuclear import by masking the nuclear localization signal (NLS) (By similarity). In contrast, binding to nuclear globular actin (G-actin) promotes nuclear export to the cytoplasm (By similarity). Nuclear localization is regulated by MICAL2, which mediates depolymerization of nuclear actin, which decreases nuclear G-actin pool, thereby promoting retention of MRTFA in the nucleus and subsequent formation of an active complex with SRF (PubMed:24440334). .
