FGF-2 Polyclonal Antibody
¥680.00
- 产品信息
商品描述
靶点-Target
eIF3ε
反应性
Human,Mouse,Rat
应用
WB,IHC,IF,ELISA
宿主物种-Host Species
Rabbit
同种型-Isotype
IgG
修饰-Modification
Unmodified
MW(Observed)
38kD
推荐稀释比
WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
浓度
1 mg/ml
克隆性
Polyclonal
免疫原
The antiserum was produced against synthesized peptide derived from human EIF3F. AA range:81-130
特异性
eIF3ε Polyclonal Antibody detects endogenous levels of eIF3ε protein.
基因名称
EIF3F
蛋白名称
Eukaryotic translation initiation factor 3 subunit F
别名
EIF3F;EIF3S5;Eukaryotic translation initiation factor 3 subunit F;eIF3f;Deubiquitinating enzyme eIF3f;Eukaryotic translation initiation factor 3 subunit 5;eIF-3-epsilon;eIF3 p47
Organism-1
Human
基因ID-1
8665
SwissProt-1
O00303
Organism-2
Mouse
基因ID-2
66085
SwissProt-2
Q9DCH4
背景
function:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.,mass spectrometry: PubMed:17322308,mass spectrometry: PubMed:18599441,PTM:Phosphorylated. Phosphorylation is enhanced upon serum stimulation.,similarity:Belongs to the eIF-3 subunit F family.,similarity:Contains 1 MPN (JAB/Mov34) domain.,subunit:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of FRAP1 and RAPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.,
细胞定位
Cytoplasm .
