FGF-2 Polyclonal Antibody
¥680.00
- 产品信息
商品描述
靶点-Target
EID-1
反应性
Human,Mouse
应用
WB,IHC,IF,ELISA
宿主物种-Host Species
Rabbit
同种型-Isotype
IgG
修饰-Modification
Unmodified
MW(Observed)
21kD
推荐稀释比
WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
浓度
1 mg/ml
克隆性
Polyclonal
免疫原
The antiserum was produced against synthesized peptide derived from human EID1. AA range:71-120
特异性
EID-1 Polyclonal Antibody detects endogenous levels of EID-1 protein.
基因名称
EID1
蛋白名称
EP300-interacting inhibitor of differentiation 1
别名
EID1;C15orf3;CRI1;RBP21;PNAS-22;PTD014;EP300-interacting inhibitor of differentiation 1;21 kDa pRb-associated protein;CREBBP/EP300 inhibitory protein 1;E1A-like inhibitor of differentiation 1;EID-1
Organism-1
Human
基因ID-1
23741
SwissProt-1
Q9Y6B2
Organism-2
Mouse
SwissProt-2
Q9DCR4
背景
developmental stage:Expression decreased with development in ventricular tissue while remaining highly expressed in adult atrial tissue. In primary cultures of human skeletal myocytes, expression decreased during myogenic differentiation (at protein level).,function:Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.,induction:Down-regulated in differentiating U937 leukemia cells.,miscellaneous:Inhibition of MYOD1 may be partly due to the ability of EID1 to bind and inhibit EP300 histone acetyltransferase activity.,PTM:Ubiquitinated in U-2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit.,subcellular location:May shuttle between nucleus and cytoplasm.,subunit:Interacts via its LXCXE motif with the entire pocket region of RB1. Interacts with EP300, NR0B2 and TRIM27.,tissue specificity:Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A549 and various leukemia cell lines.,
细胞定位
Nucleus . Cytoplasm . May shuttle between nucleus and cytoplasm. .
