FGF-2 Polyclonal Antibody
¥680.00
Smad2/3 (phospho Thr8) Polyclonal Antibody
活动价：
¥680.00
市场价：¥880.00
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产品规格：
- 50μL
- 100μL
货号：
FAB2611143
- 产品信息
商品描述
靶点-Target
Smad2/3
反应性
Human,Mouse,Rat,Pig
应用
WB,IF,ELISA
宿主物种-Host Species
Rabbit
同种型-Isotype
IgG
修饰-Modification
Phospho
MW(Observed)
48kD
推荐稀释比
WB 1:500-2000;IF/ICC1:100-500;ELISA 1:5000-20000
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
浓度
1 mg/ml
克隆性
Polyclonal
免疫原
The antiserum was produced against synthesized peptide derived from human Smad2/3 around the phosphorylation site of Thr8. AA range:1-50
特异性
Phospho-Smad2/3 (T8) Polyclonal Antibody detects endogenous levels of Smad2/3 protein only when phosphorylated at T8.
基因名称
SMAD2/SMAD3
蛋白名称
Mothers against decapentaplegic homolog 2/3
别名
SMAD2;MADH2;MADR2;Mothers against decapentaplegic homolog 2;MAD homolog 2;Mothers against DPP homolog 2;JV18-1;Mad-related protein 2;hMAD-2;SMAD family member 2;SMAD 2;Smad2;hSMAD2;SMAD3;MADH3;Mothers against decapentaplegic
Organism-1
Human
基因ID-1
4087;4088
SwissProt-1
Q15796;P84022
Organism-2
Mouse
基因ID-2
17126;17127
Organism-3
Rat
基因ID-3
29357;25631
SwissProt-3
O70436;P84025
背景
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation
细胞定位
Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:9865696, PubMed:21145499). On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm at the blastocyst and epiblast stages (By similarity). .
