FGF-2 Polyclonal Antibody
¥680.00
Raf-1 (phospho Ser259) Polyclonal Antibody
活动价：
¥680.00
市场价：¥880.00
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产品规格：
- 50μL
- 100μL
货号：
FAB2611020
- 产品信息
商品描述
靶点-Target
Raf-1
反应性
Human,Mouse,Rat,Monkey
应用
WB,IHC,IF,ELISA
宿主物种-Host Species
Rabbit
同种型-Isotype
IgG
修饰-Modification
Phospho
MW(Observed)
73kD
推荐稀释比
WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
浓度
1 mg/ml
克隆性
Polyclonal
免疫原
The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser259. AA range:225-274
特异性
Phospho-Raf-1 (S259) Polyclonal Antibody detects endogenous levels of Raf-1 protein only when phosphorylated at S259.
基因名称
RAF1
蛋白名称
RAF proto-oncogene serine/threonine-protein kinase
别名
RAF1;RAF;RAF proto-oncogene serine/threonine-protein kinase;Proto-oncogene c-RAF;cRaf;Raf-1
Organism-1
Human
基因ID-1
5895
SwissProt-1
P04049
Organism-2
Mouse
基因ID-2
110157
SwissProt-2
Q99N57
Organism-3
Rat
基因ID-3
24703
SwissProt-3
P11345
背景
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008],
细胞定位
Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus.
