FGF-2 Polyclonal Antibody
¥680.00
- 产品信息
商品描述
靶点-Target
MBP-Tag
反应性
Species independent
应用
WB
宿主物种-Host Species
Rabbit
同种型-Isotype
IgG
修饰-Modification
Unmodified
推荐稀释比
WB 1:5000
组成
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
克隆性
Polyclonal
免疫原
Recombinant Protein of MBP-Tag
特异性
The antibody detects MBP & MBP tag fusion proteins.
别名
YM3209
背景
Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose.
