DDDDK-Tag(binds to flag sequnence) mouse mAb(12M11)
活动价:
市场价:¥1280.00
- 50μL
FAB2601662
- 产品信息
商品描述
靶点-Target
Flag-tag
反应性
Species independent
应用
WB,ELISA,IP,IF,CoIP
宿主物种-Host Species
Mouse
同种型-Isotype
VH IgG1 and VL Kappa
修饰-Modification
Unmodified
推荐稀释比
WB 1:10000-50000;ELISA 1:20000-50000;IP 1:50-1:200;IF 1:1000 -3000
组成
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
来源
Mouse VH IgG1 and VL Kappa was expressed from 293F cells
纯化工艺
Protein A purified
纯度
>95% SDS-PAGE
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
克隆性
Monoclonal
克隆号
12M11
免疫原
Synthetic Peptide of Flag-Tag
特异性
This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library immunized with DDDK peptide. Constant region of this antibody is mouse IgG1 and mouse kappa.
基因名称
Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag
背景
The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.





