FGF-2 Polyclonal Antibody
¥680.00
- 产品信息
商品描述
靶点-Target
PLK1
反应性
Human,Mouse,Rat
应用
IHC,WB
宿主物种-Host Species
Mouse
修饰-Modification
Unmodified
MW(Observed)
62kD
推荐稀释比
IHC1:50-200;WB 1:1000-2000
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
浓度
1 mg/ml
克隆性
Monoclonal
免疫原
Synthesized peptide derived from human PLK1
特异性
This antibody detects endogenous levels of PLK1 at Human, Mouse,Rat
基因名称
PLK1 PLK
蛋白名称
PLK1
别名
Serine/threonine-protein kinase PLK1;Polo-like kinase 1;PLK-1;Serine/threonine-protein kinase 13;STPK13;
Organism-1
Human
基因ID-1
5347
SwissProt-1
P53350
Organism-2
Mouse
基因ID-2
18817
SwissProt-2
Q07832
Organism-3
Rat
基因ID-3
25515
SwissProt-3
Q62673
背景
The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015],
细胞定位
Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Midbody . localization at the centrosome starts at the G1/S transition (PubMed:24018379). During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGO1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization. Localizes onto the central spindle by phosphorylating and docking at midzone proteins KIF20A/MKLP2 and PRC1. Colocalizes with FRY to separating centrosomes and spindle poles from prophase to metaphase in mitosis, but not in other stages of the cell cycle. Localization to the centrosome is required for S phase progression (PubMed:24018379). Colocalizes with HSF1 at the spindle poles during prometaphase (PubMed:18794143). .
