FGF-2 Polyclonal Antibody
¥680.00
- 产品信息
商品描述
靶点-Target
EYFP
反应性
Species independent
应用
WB,IF
宿主物种-Host Species
Mouse
修饰-Modification
Unmodified
推荐稀释比
WB 1:5000;IF 1:300-500
组成
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
纯化工艺
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
克隆性
Monoclonal
免疫原
Recombinant Protein of EYFP
特异性
The antibody detects EYFP YFP CFP morange tag fusion proteins. It is also reactive against GFP tag weakly
背景
Enhanced Yellow florescent protein.Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514nm and its emission peak is 527nm. Like green fluorescent protein (GFP), it is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the extinction coefficient and quantum yield). Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore.
