FGF-2 Polyclonal Antibody
¥680.00
- 产品信息
商品描述
靶点-Target
DDX3
反应性
Human,Rat,Mouse,Monkey
应用
WB,IP,ICC
宿主物种-Host Species
Mouse
修饰-Modification
Unmodified
MW(Observed)
75kD
推荐稀释比
WB 1:1000;ICC 1:200
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
克隆性
Monoclonal
免疫原
Purified recombinant human DDX3 protein fragments expressed in E.coli.
特异性
This antibody detects endogenous levels of DDX3 and does not cross-react with related proteins.
基因名称
ddx3x
别名
ATP dependent RNA helicase DDX3X;ATP-dependent RNA helicase DDX3X;CAP Rf;DBX;DDX14;DDX3X;DDX3X_HUMAN;DEAD;Asp Glu Ala Asp;box polypeptide 3 X linked;DEAD box;DEAD box protein 3;DEAD box protein 3 X-chromosomal;DEAD box X isoform;DEAD/H;Asp Glu Ala Asp/His;box polypeptide 3;DEAD/H box 3;DEAD/H box 3, X-linked;Fibroblast Growth Factor Inducible 14;Fin14;Helicase like protein 2;Helicase-like protein 2;HLP2;X isoform;X-chromosomal.
Organism-1
Human
基因ID-1
1654
SwissProt-1
O00571
Organism-2
Mouse
SwissProt-2
Q62167
背景
The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarit
细胞定位
Cell membrane . Nucleus . Cytoplasm . Cytoplasm, Stress granule . Inflammasome . Cell projection, lamellipodium . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Shuttles between the nucleus and the cytosol (PubMed:15507209, PubMed:18636090, PubMed:29899501, PubMed:31575075, PubMed:30131165). Exported from the nucleus partly through the XPO1/CRM1 system and partly through NXF1/TAP (PubMed:15507209, PubMed:18636090, PubMed:18596238, PubMed:31575075, PubMed:30131165). Localizes to nuclear pores on the outer side of the nuclear membrane (PubMed:15507209). In the cytosol, partly colocalizes with mitochondria (PubMed:20127681). At G0, predominantly located in nucleus. In G1/S phase, predominantly cytoplasmic (PubMed:22034099). During prophase/prometaphase, localizes in close proximity to the condensing chromosomes (PubMed:30131165, PubMed:21730191). During telophase, localizes around the newly synthesized nuclear membrane and in the cytoplasm (PubMed:22034099). Colocalizes with TRPV4 at the plasma membrane. When TRPV4 channel is activated, intracellular Ca(2+) levels increase and the calmodulin/CAMKII pathway is activated, relocalizes to the nucleus (PubMed:29899501). WNT3A stimulation promotes DDX3 recruitment to the plasma membrane (PubMed:23413191). At the leading edge of migrating fibroblasts, colocalizes with CAPRIN1 and PABPC1 (PubMed:28733330). Localizes to centrosome throughout the cell cycle and associates with TP53 at centrosome during mitosis (PubMed:28842590). Translocates to the nucleus in response to HPIV-3 virus-mediated infection (PubMed:31575075). .
