FGF-2 Polyclonal Antibody
¥680.00
- 产品信息
商品描述
靶点-Target
FEN-1
反应性
Human,Mouse,Rat,Monkey,Hamster
应用
WB,ICC
宿主物种-Host Species
Mouse
修饰-Modification
Unmodified
MW(Observed)
45kD
推荐稀释比
WB 1:1000;ICC 1:400
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
克隆性
Monoclonal
免疫原
Purified recombinant human FEN-1 protein fragments expressed in E.coli.
特异性
This antibody detects endogenous levels of FEN-1 and does not cross-react with related proteins.
基因名称
fen1
别名
DNase IV;FEN-1;FEN1;FEN1_HUMAN;Flap endonuclease 1;Flap structure specific endonuclease 1;Flap structure-specific endonuclease 1;hFEN-1;hFEN1;Maturation factor 1;MF1;Rad2.
Organism-1
Human
基因ID-1
2237
SwissProt-1
P39748
Organism-2
Mouse
SwissProt-2
P39749
背景
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions
细胞定位
[Isoform 1]: Nucleus, nucleolus. Nucleus, nucleoplasm. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.; [Isoform FENMIT]: Mitochondrion .
