FGF-2 Polyclonal Antibody
¥680.00
- 产品信息
商品描述
靶点-Target
FoxO1
反应性
Human,Mouse
应用
WB,IF
宿主物种-Host Species
Mouse
修饰-Modification
Unmodified
MW(Calculated)
70kD
推荐稀释比
WB 1:1000-1:2000;IF 1:100-1:500;Not yet tested in other applications.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
Affinity purification
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
克隆性
Monoclonal
免疫原
Purified recombinant human FoxO1A (C-terminus) protein fragments expressed in E.coli.
特异性
FoxO1A Monoclonal Antibody detects endogenous levels of FoxO1A protein.
基因名称
FOXO1
蛋白名称
Forkhead box protein O1
别名
FOXO1;FKHR;FOXO1A;Forkhead box protein O1;Forkhead box protein O1A;Forkhead in rhabdomyosarcoma
Organism-1
Human
基因ID-1
2308
SwissProt-1
Q12778
Organism-2
Mouse
基因ID-2
56458
SwissProt-2
Q9R1E0
背景
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008],
细胞定位
Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells (PubMed:11311120, PubMed:12228231, PubMed:19221179, PubMed:21245099, PubMed:20543840, PubMed:25009184). In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Serum deprivation increases localization to the nucleus, leading to activate expression of SOX9 and subsequent chondrogenesis (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway (PubMed:11237865, PubMed:12228231). Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation (PubMed:18356527). Phosphorylation by NLK results in nuclear export (By similarity). Translocates to the nucleus upon oxidative stress-induced phosphorylation at Ser-212 by STK4/MST1 (PubMed:19221179, PubMed:21245099). SGK1-mediated phosphorylation also results in nuclear translocation (By similarity). Retained in the nucleus under stress stimuli including oxidative stress, nutrient deprivation or nitric oxide (By similarity). Retained in the nucleus on methylation (By similarity). PPIA/CYPA stimulates its nuclear accumulation (PubMed:31063815). Deacetylation by SIRT6, promotes its translocation into the cytoplasm (PubMed:25009184). .
