FGF-2 Polyclonal Antibody
¥680.00
- 产品信息
商品描述
靶点-Target
Dynactin 1
反应性
Human,Mouse,Bovine,Pig
应用
WB,IF
宿主物种-Host Species
Mouse
修饰-Modification
Unmodified
MW(Calculated)
142kD
推荐稀释比
WB 1:1000-1:2000;IF 1:100-1:500;Not yet tested in other applications.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
Affinity purification
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
克隆性
Monoclonal
免疫原
Purified recombinant human Dynactin 1 protein fragments expressed in E.coli.
特异性
Dynactin 1 Monoclonal Antibody detects endogenous levels of Dynactin 1 protein.
基因名称
DCTN1
蛋白名称
Dynactin subunit 1
别名
DCTN1;Dynactin subunit 1;150 kDa dynein-associated polypeptide;DAP-150;DP-150;p135;p150-glued
Organism-1
Human
基因ID-1
1639
SwissProt-1
Q14203
Organism-2
Mouse
基因ID-2
13191
SwissProt-2
O08788
背景
This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [
细胞定位
Cytoplasm . Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Cytoplasm, cytoskeleton, spindle . Nucleus envelope . Cytoplasm, cell cortex . Localizes to microtubule plus ends (PubMed:17828277, PubMed:22777741, PubMed:25774020). Localizes preferentially to the ends of tyrosinated microtubules (PubMed:26972003). Localization at centrosome is regulated by SLK-dependent phosphorylation (PubMed:23985322). Localizes to centrosome in a PARKDA-dependent manner (PubMed:20719959). Localizes to the subdistal appendage region of the centriole in a KIF3A-dependent manner (PubMed:23386061). PLK1-mediated phosphorylation at Ser-179 is essential for its localization in the nuclear envelope (PubMed:20679239). .
