FGF-2 Polyclonal Antibody
¥680.00
- 产品信息
商品描述
靶点-Target
Akt1
反应性
Human,Mouse,Rat,Bovine,Chicken,Pig
应用
WB
宿主物种-Host Species
Mouse
修饰-Modification
Unmodified
MW(Observed)
56kD
推荐稀释比
WB 1:1000-1:2000;Not yet tested in other applications.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
Affinity purification
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
克隆性
Monoclonal
免疫原
Purified recombinant human Akt1 (C-terminus) protein fragments expressed in E.coli.
特异性
Akt1 Monoclonal Antibody detects endogenous levels of Akt1 protein.
基因名称
AKT1
蛋白名称
RAC-alpha serine/threonine-protein kinase
别名
AKT1;PKB;RAC;RAC-alpha serine/threonine-protein kinase;Protein kinase B;PKB;Protein kinase B alpha;PKB alpha;Proto-oncogene c-Akt;RAC-PK-alpha
Organism-1
Human
基因ID-1
207
SwissProt-1
P31749
Organism-2
Mouse
基因ID-2
11651
SwissProt-2
P31750
Organism-3
Rat
基因ID-3
24185
SwissProt-3
P47196
背景
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
细胞定位
Cytoplasm . Nucleus . Cell membrane . Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles (PubMed:16792529). .
